TSA1/YML028W Protein Information 
| Standard Name | Tsa1p |
|---|---|
| Systematic Name | Yml028wp |
| Alias | Zrg14p 1 , Tpx1p |
| ORF Classification | Verified |
| Description | Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; required for telomere length maintenace; TSA1 has a paralog, TSA2, that arose from the whole genome duplication (10, 11, 2, 3, 4, 5, 6, 7, 8, 9) |
| Name Description | Thiol-Specific Antioxidant 3 |
| Gene Product | cTPxI |
| Experimental Data | |
|---|---|
| Molecules/cell | 378000 12 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Tsa1p (InterPro) |
| Transmembrane Domains | There are 1 total predicted transmembrane domains (TMHMM) |
| Physical Interactions | There are 55 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI000002FC87 | P34760 MIPS: YML028W NCBI: 349196 | 400260528 | 400260529 | 400260530 | 400260531 | 400260532 | 400260533 | 400260534 | 400260535 | 400260536 | 400260537 | 464970 | 575691 | 6323613 | NP_013684.1 GenBank/EMBL/DDBJ: DAA09870.1 | L14640 | Z46659 |
| External Classifications | EC: 1.11.1.15 [Peroxiredoxin] |
external links for Tsa1p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | Organelle DB |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YPL+ |
| Candida (CGD) | BioPIXIE | MIPS | YeastGFP |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | YeastRC Public Image Repository |
| Candida (CandidaDB) | Complexome | YeastRC Structure Prediction (Seattle) | |
| YGOB | DIP | ||
| YOGY | GeneMANIA | ||
| YeastRC Mass Spec (Seattle) |
References cited on this page View Complete Literature Guide for Tsa1p
| 1) | Yuan DS (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58 |
| 2) | Chae HZ, et al. (1994) Thioredoxin-dependent peroxide reductase from yeast. J Biol Chem 269(44):27670-8 |
| 3) | Chae HZ, et al. (1993) Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae. J Biol Chem 268(22):16815-21 |
| 4) | Wong CM, et al. (2002) Cooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress. J Biol Chem 277(7):5385-94 |
| 5) | Huang ME, et al. (2003) A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations. Proc Natl Acad Sci U S A 100(20):11529-34 |
| 6) | Wong CM, et al. (2004) Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and are genomically unstable. J Biol Chem 279(22):23207-13 |
| 7) | Okazaki S, et al. (2005) Peroxiredoxin-mediated redox regulation of the nuclear localization of Yap1, a transcription factor in budding yeast. Antioxid Redox Signal 7(3-4):327-34 |
| 8) | Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61 |
| 9) | Trotter EW, et al. (2008) The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J 412(1):73-80 |
| 10) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
| 11) | Lu J, et al. (2013) Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis. Aging Cell () |
| 12) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
