TRM9/YML014W Protein Information

Standard Name	Trm9p
Systematic Name	Yml014wp
Alias	Kti1p 1
ORF Classification	Verified
Description	tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses (2, 3, 4, 5)
Name Description	TRna Methyltransferase 2

Experimental Data
Molecules/cell	2360 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	279
Molecular Weight (Da)	32,438
Isoelectric Point (pI)	8.51

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trm9p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052EEC \| P49957 MIPS: YML014W NCBI: 1730616 \| 6323627 \| 854476 \| NP_013698.1 \| NM_001182372.1 GenBank/EMBL/DDBJ: DAA09884.1 \| Z49810
External Classifications	EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases] EC: 2.1.1.229 [tRNA (carboxymethyluridine(34)-5-O)-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MEINQAAEKE QEYVHKVYNE IAPHFSQTRY KPWPIVTQFL KTRPMGSIGI 51 DVGCGNGKYL GVNPDIYIIG SDRSDGLIEC ARGINPSYNL LVADGLNLPH 101 KNETFDFAIS IAVVHHWSTR ERRVEVIRHV LSKLRQGGQA LIYCWALEQG 151 SSRRGYHEGM EQDVFVPWVL PKSKSKPKTK STPPAKVKTR PKPNLMNIPP 201 KERSEYLQRW KEEQQRSKSL DDNDEKQQQD QEQEREEVKY RYYHLYREGE 251 LAEDCRQAGA AVHSEGFERD NWWVVAQKR*

Amino Acid Sequence (or in FASTA format)

       1  MEINQAAEKE QEYVHKVYNE IAPHFSQTRY KPWPIVTQFL KTRPMGSIGI
      51  DVGCGNGKYL GVNPDIYIIG SDRSDGLIEC ARGINPSYNL LVADGLNLPH
     101  KNETFDFAIS IAVVHHWSTR ERRVEVIRHV LSKLRQGGQA LIYCWALEQG
     151  SSRRGYHEGM EQDVFVPWVL PKSKSKPKTK STPPAKVKTR PKPNLMNIPP
     201  KERSEYLQRW KEEQQRSKSL DDNDEKQQQD QEQEREEVKY RYYHLYREGE
     251  LAEDCRQAGA AVHSEGFERD NWWVVAQKR*

external links for Trm9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Trm9p

1)	Butler AR, et al. (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25
2)	Kalhor HR and Clarke S (2003) Novel methyltransferase for modified uridine residues at the wobble position of tRNA. Mol Cell Biol 23(24):9283-92
3)	Jablonowski D, et al. (2006) tRNAGlu wobble uridine methylation by Trm9 identifies Elongator's key role for zymocin-induced cell death in yeast. Mol Microbiol 59(2):677-88
4)	Studte P, et al. (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77
5)	Patil A, et al. (2012) Translational infidelity-induced protein stress results from a deficiency in Trm9-catalyzed tRNA modifications. RNA Biol 9(7):990-1001
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41