SPT5/YML010W Protein Information 
| Standard Name | Spt5p 1 |
|---|---|
| Systematic Name | Yml010wp |
| ORF Classification | Verified |
| Description | Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair (10, 2, 3, 4, 5, 6, 7, 8, 9) |
| Name Description | SuPpressor of Ty's 1 |
| Experimental Data | |
|---|---|
| Molecules/cell | 2340 11 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Spt5p (InterPro) |
| Physical Interactions | There are 257 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI0000053123 | P27692 MIPS: YML010W NCBI: 119389383 | 134853 | 172680 | 6323632 | 854480 | NP_013703.1 GenBank/EMBL/DDBJ: DAA09888.1 | M62882 | Z49810 |
external links for Spt5p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | YPL+ |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YeastGFP |
| Candida (CGD) | BioPIXIE | MIPS | YeastRC Public Image Repository |
| Candida (CandidaDB) | CYC2008 (complexes) | Pfam domains | |
| YGOB | Complexome | YeastRC Structure Prediction (Seattle) | |
| YOGY | DIP | ||
| GeneMANIA |
References cited on this page View Complete Literature Guide for Spt5p
| 1) | Winston F, et al. (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97 |
| 2) | Lindstrom DL, et al. (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78 |
| 3) | Rondon AG, et al. (2003) Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22(3):612-20 |
| 4) | Schneider DA, et al. (2006) RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A 103(34):12707-12 |
| 5) | Zhou K, et al. (2009) Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5. Proc Natl Acad Sci U S A 106(17):6956-61 |
| 6) | Liu Y, et al. (2009) Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol 29(17):4852-63 |
| 7) | Ding B, et al. (2010) The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J Biol Chem 285(8):5317-26 |
| 8) | Shen Z, et al. (2010) Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. Genes Dev 24(17):1914-26 |
| 9) | Anderson SJ, et al. (2011) The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively. J Biol Chem 286(21):18816-24 |
| 10) | Lepore N and Lafontaine DL (2011) A Functional Interface at the rDNA Connects rRNA Synthesis, Pre-rRNA Processing and Nucleolar Surveillance in Budding Yeast. PLoS One 6(9):e24962 |
| 11) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
