YAP1/YML007W Protein Information

Standard Name	Yap1p 1
Systematic Name	Yml007wp
Alias	Par1p , Snq3p
ORF Classification	Verified
Description	Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress (2, 3, 4, 5, 6, 7, 8)
Name Description	Yeast AP-1 2

Experimental Data
Molecules/cell	1620 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	650
Molecular Weight (Da)	72,532
Isoelectric Point (pI)	4.64

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yap1p (InterPro)
Physical Interactions	There are 132 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052EC3 \| P19880 MIPS: YML007W NCBI: 253722373 \| 253722374 \| 4123 \| 417456 \| 4506 \| 4798 \| 499193 \| 6323636 \| 854483 \| NP_013707.1 GenBank/EMBL/DDBJ: DAA09892.1 \| X53830 \| X58693 \| X60780 \| X63268 \| Z49810

Amino Acid Sequence (or in FASTA format)
1 MSVSTAKRSL DVVSPGSLAE FEGSKSRHDE IENEHRRTGT RDGEDSEQPK 51 KKGSKTSKKQ DLDPETKQKR TAQNRAAQRA FRERKERKMK ELEKKVQSLE 101 SIQQQNEVEA TFLRDQLITL VNELKKYRPE TRNDSKVLEY LARRDPNLHF 151 SKNNVNHSNS EPIDTPNDDI QENVKQKMNF TFQYPLDNDN DNDNSKNVGK 201 QLPSPNDPSH SAPMPINQTQ KKLSDATDSS SATLDSLSNS NDVLNNTPNS 251 STSMDWLDNV IYTNRFVSGD DGSNSKTKNL DSNMFSNDFN FENQFDEQVS 301 EFCSKMNQVC GTRQCPIPKK PISALDKEVF ASSSILSSNS PALTNTWESH 351 SNITDNTPAN VIATDATKYE NSFSGFGRLG FDMSANHYVV NDNSTGSTDS 401 TGSTGNKNKK NNNNSDDVLP FISESPFDMN QVTNFFSPGS TGIGNNAASN 451 TNPSLLQSSK EDIPFINANL AFPDDNSTNI QLQPFSESQS QNKFDYDMFF 501 RDSSKEGNNL FGEFLEDDDD DKKAANMSDD ESSLIKNQLI NEEPELPKQY 551 LQSVPGNESE ISQKNGSSLQ NADKINNGND NDNDNDVVPS KEGSLLRCSE 601 IWDRITTHPK YSDIDVDGLC SELMAKAKCS ERGVVINAED VQLALNKHMN 651 *

Amino Acid Sequence (or in FASTA format)

       1  MSVSTAKRSL DVVSPGSLAE FEGSKSRHDE IENEHRRTGT RDGEDSEQPK
      51  KKGSKTSKKQ DLDPETKQKR TAQNRAAQRA FRERKERKMK ELEKKVQSLE
     101  SIQQQNEVEA TFLRDQLITL VNELKKYRPE TRNDSKVLEY LARRDPNLHF
     151  SKNNVNHSNS EPIDTPNDDI QENVKQKMNF TFQYPLDNDN DNDNSKNVGK
     201  QLPSPNDPSH SAPMPINQTQ KKLSDATDSS SATLDSLSNS NDVLNNTPNS
     251  STSMDWLDNV IYTNRFVSGD DGSNSKTKNL DSNMFSNDFN FENQFDEQVS
     301  EFCSKMNQVC GTRQCPIPKK PISALDKEVF ASSSILSSNS PALTNTWESH
     351  SNITDNTPAN VIATDATKYE NSFSGFGRLG FDMSANHYVV NDNSTGSTDS
     401  TGSTGNKNKK NNNNSDDVLP FISESPFDMN QVTNFFSPGS TGIGNNAASN
     451  TNPSLLQSSK EDIPFINANL AFPDDNSTNI QLQPFSESQS QNKFDYDMFF
     501  RDSSKEGNNL FGEFLEDDDD DKKAANMSDD ESSLIKNQLI NEEPELPKQY
     551  LQSVPGNESE ISQKNGSSLQ NADKINNGND NDNDNDVVPS KEGSLLRCSE
     601  IWDRITTHPK YSDIDVDGLC SELMAKAKCS ERGVVINAED VQLALNKHMN
     651  *

external links for Yap1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Yap1p

1)	Preston, R. and Jones, E. (1989) Personal Communication, Mortimer Map Edition 10
2)	Moye-Rowley WS, et al. (1989) Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins. Genes Dev 3(3):283-92
3)	Wu A, et al. (1993) Yeast bZip proteins mediate pleiotropic drug and metal resistance. J Biol Chem 268(25):18850-8
4)	Kuge S, et al. (1997) Regulation of yAP-1 nuclear localization in response to oxidative stress. EMBO J 16(7):1710-20
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Okazaki S, et al. (2007) Multistep disulfide bond formation in Yap1 is required for sensing and transduction of H(2)O(2) stress signal. Mol Cell 27(4):675-88
7)	Gulshan K, et al. (2012) Proteolytic degradation of the Yap1 transcription factor is regulated by subcellular localization and the E3 ubiquitin ligase Not4. J Biol Chem 287(32):26796-805
8)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41