TRM12/YML005W Protein Information

Standard Name	Trm12p 1
Systematic Name	Yml005wp
Alias	Tyw2p 2
ORF Classification	Verified
Description	S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA (1)
Name Description	TRna Methyltransferase 1

Experimental Data
Molecules/cell	656 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	462
Molecular Weight (Da)	52,726
Isoelectric Point (pI)	7.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trm12p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B967 \| Q04235 MIPS: YML005W NCBI: 2497109 \| 6323638 \| 854485 \| NP_013709.1 GenBank/EMBL/DDBJ: DAA09894.1 \| Z49810
External Classifications	EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases] EC: 2.5.1.- [Transferases transferring alkyl or aryl groups, other than methyl groups]

Amino Acid Sequence (or in FASTA format)
1 MSDETMPVEF LVSDKRLLKT IKVKLETNGL FVTPIYSDND NKVIKSSIED 51 LNHPLAVEIN NIAGVKARFH ESGNLERSEG HLKHQSNSIT EFTKSFLKDH 101 GLANDKIFLS HLLDHLPLKY TIYPPVVLFN NSTVRSFNHP IWQKAFQLKL 151 FDPNEYYREL LCFLSPGKPS KGTSLHPNNR LLTHLAINNP ITEADVLRRP 201 FNIQPLYGKL IDDSILDDND NTLWENPSQE QLNSSIWCKV IQNGVTQIWS 251 PVFTMFSRGN IKEKKRVLTT FPDICNNDVV DLYAGIGYFT FSYLTKGART 301 LFAFELNPWS VEGLKRGLKA NGFNKSGNCH VFQESNEMCV QRLTEFLSQN 351 PGFRLRIRHI NLGLLPSSKQ GWPLAIKLIY LQGASLEKVT MHIHENVHID 401 AIEDGSFEKN VIVELDAINE SIALIRNRGI KLQFVRSKLE RIKTFAPDIW 451 HVCVDVDVIV ST*

Amino Acid Sequence (or in FASTA format)

       1  MSDETMPVEF LVSDKRLLKT IKVKLETNGL FVTPIYSDND NKVIKSSIED
      51  LNHPLAVEIN NIAGVKARFH ESGNLERSEG HLKHQSNSIT EFTKSFLKDH
     101  GLANDKIFLS HLLDHLPLKY TIYPPVVLFN NSTVRSFNHP IWQKAFQLKL
     151  FDPNEYYREL LCFLSPGKPS KGTSLHPNNR LLTHLAINNP ITEADVLRRP
     201  FNIQPLYGKL IDDSILDDND NTLWENPSQE QLNSSIWCKV IQNGVTQIWS
     251  PVFTMFSRGN IKEKKRVLTT FPDICNNDVV DLYAGIGYFT FSYLTKGART
     301  LFAFELNPWS VEGLKRGLKA NGFNKSGNCH VFQESNEMCV QRLTEFLSQN
     351  PGFRLRIRHI NLGLLPSSKQ GWPLAIKLIY LQGASLEKVT MHIHENVHID
     401  AIEDGSFEKN VIVELDAINE SIALIRNRGI KLQFVRSKLE RIKTFAPDIW
     451  HVCVDVDVIV ST*

external links for Trm12p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Trm12p

1)	Kalhor HR, et al. (2005) A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA. Biochem Biophys Res Commun 334(2):433-40
2)	Noma A, et al. (2006) Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J 25(10):2142-54
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41