RIF2/YLR453C Protein Information

Standard Name	Rif2p
Systematic Name	Ylr453cp
ORF Classification	Verified
Description	Protein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Rap1p-Interacting Factor 5

Experimental Data
Molecules/cell	589 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	395
Molecular Weight (Da)	45,643
Isoelectric Point (pI)	9.9

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rif2p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001338EF \| Q06208 MIPS: YLR453C NCBI: 27805711 \| 398366349 \| 51013171 \| 717069 \| NP_013558.3 GenBank/EMBL/DDBJ: DAA09753.1 \| AY692860 \| U22382

Amino Acid Sequence (or in FASTA format)
1 MEHVDSDFAP IRRSKKVVDS DKIVKAISDD LEQKNFTVLR KLNLVPIKKS 51 VSSPKVCKPS PVKERVDHVF YQKFKSMALQ ELGTNYLSIS YVPSLSKFLS 101 KNLRSMKNCI VFFDKVEHIH QYAGIDRAVS ETLSLVDINV VIIEMNDYLM 151 KEGIQSSKSK ECIESMGQAS YSGQLDFEAS EKPSNHTSDL MMMVMRKINN 201 DESIDHIVYF KFEQLDKLST STIIEPSKLT EFINVLSVLE KSNNIAFKVL 251 IYSNNVSISS LLSTSLKKKL NTKYTVFEMP ILTCAQEQEY LKKMIKFTFD 301 SGSKLLQSYN SLVTCQLNNK ESNLAIFFEF LKVFPHPFTY LFNAYTEIIV 351 QSRTFDELLD KIRNRLTIKN YPHSAYNFKK NQRLPLKLTR KVHDR*

Amino Acid Sequence (or in FASTA format)

       1  MEHVDSDFAP IRRSKKVVDS DKIVKAISDD LEQKNFTVLR KLNLVPIKKS
      51  VSSPKVCKPS PVKERVDHVF YQKFKSMALQ ELGTNYLSIS YVPSLSKFLS
     101  KNLRSMKNCI VFFDKVEHIH QYAGIDRAVS ETLSLVDINV VIIEMNDYLM
     151  KEGIQSSKSK ECIESMGQAS YSGQLDFEAS EKPSNHTSDL MMMVMRKINN
     201  DESIDHIVYF KFEQLDKLST STIIEPSKLT EFINVLSVLE KSNNIAFKVL
     251  IYSNNVSISS LLSTSLKKKL NTKYTVFEMP ILTCAQEQEY LKKMIKFTFD
     301  SGSKLLQSYN SLVTCQLNNK ESNLAIFFEF LKVFPHPFTY LFNAYTEIIV
     351  QSRTFDELLD KIRNRLTIKN YPHSAYNFKK NQRLPLKLTR KVHDR*

external links for Rif2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rif2p

1)	Wotton D and Shore D (1997) A novel Rap1p-interacting factor, Rif2p, cooperates with Rif1p to regulate telomere length in Saccharomyces cerevisiae. Genes Dev 11(6):748-60
2)	Teixeira MT, et al. (2004) Telomere length homeostasis is achieved via a switch between telomerase- extendible and -nonextendible states. Cell 117(3):323-35
3)	Smogorzewska A and de Lange T (2004) Regulation of telomerase by telomeric proteins. Annu Rev Biochem 73:177-208
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Hardy CF, et al. (1992) A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes Dev 6(5):801-14
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41