LEU3/YLR451W Protein Information

Standard Name	Leu3p
Systematic Name	Ylr451wp
ORF Classification	Verified
Description	Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation (1, 2, 3, 4, 5)
Name Description	LEUcine biosynthesis 6

Experimental Data
Molecules/cell	125 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	886
Molecular Weight (Da)	100,152
Isoelectric Point (pI)	5.99

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Leu3p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012E5AE \| P08638 MIPS: YLR451W NCBI: 126217 \| 171833 \| 3873 \| 398366339 \| 717060 \| 93279244 \| 93279245 \| 93279246 \| 93279247 \| 93279252 \| 93279253 \| 93279256 \| 93279257 \| NP_013556.3 GenBank/EMBL/DDBJ: DAA09751.1 \| M17222 \| U22382 \| Y00360

Amino Acid Sequence (or in FASTA format)
1 MEGRSDFVAT SQSGSEMSHS ETRNRTGMNA RKRKFACVEC RQQKSKCDAH 51 ERAPEPCTKC AKKNVPCILK RDFRRTYKRA RNEAIEKRFK ELTRTLTNLT 101 SDEILKKIEE EQEIVLDNSN FTKEKVKQLR KSAFETTEIE PRSYKTLRGE 151 PISYSTNRRH TDSSPLTLLS SSTNFDPVHS TNVMTDDQLK CLPKSLGDVY 201 LSSSDIAELF QEFATKYHQF LPVVDLSKGA ERIYHLSPCL FWVILLIGLR 251 RKFGATDLMT RLSVLVKSVL SEITISPIIR YTPSDKDEPV LNVASVYSVQ 301 AFLLYTFWPP LTSSLSADTS WNTIGTAMFQ ALRVGLNCAG FSKEYASANS 351 ELVNEQIRTW ICCNVVSQTV ASSFGFPAYV SFDYLVISSI RVPNSKSQVD 401 IPNELRQMAQ IARFENQIVN TMNSTPASVT GMVSQEEKQP LLHVLNQQLS 451 QLEISLEENN LDDIRKFLLL VAKVHLLTYY FTDVTSQSAG KSNGNIYEGS 501 YSIMELDTSF ETKRGLVKVY NAAVNFLIHA NSMWEHDPTI IKYFPGLFVL 551 NIWQSACIIS KLIHSSLHSM LDVNSGKKAY NNAISLTFNA SVLKYDMAYR 601 SSGIMRSIWS LFANMYDAWK NDQKEGGGRL NNDFNLGITI KSRMSVNVFF 651 DCLYILKEKC GMAKLERETK VSTAYNVDEE EEEDEDEEGE EEEEEEELSS 701 KVPENMDSQQ LRTRKFTNVR HPEKKARKII ETIPLDPNPI NAGSTSSGSS 751 LTTPNSQVAN TISYRGILNK MSPREQLNHA NLDSSVSTDI KDTEAVNEPL 801 PIGRNAEHPA NQPPLSITQM QENTLPATQA NSSLLETYPI VQSNPVTTTI 851 KESPNSIMAG WDNWESDMVW RDVDILMNEF AFNPKV*

Amino Acid Sequence (or in FASTA format)

       1  MEGRSDFVAT SQSGSEMSHS ETRNRTGMNA RKRKFACVEC RQQKSKCDAH
      51  ERAPEPCTKC AKKNVPCILK RDFRRTYKRA RNEAIEKRFK ELTRTLTNLT
     101  SDEILKKIEE EQEIVLDNSN FTKEKVKQLR KSAFETTEIE PRSYKTLRGE
     151  PISYSTNRRH TDSSPLTLLS SSTNFDPVHS TNVMTDDQLK CLPKSLGDVY
     201  LSSSDIAELF QEFATKYHQF LPVVDLSKGA ERIYHLSPCL FWVILLIGLR
     251  RKFGATDLMT RLSVLVKSVL SEITISPIIR YTPSDKDEPV LNVASVYSVQ
     301  AFLLYTFWPP LTSSLSADTS WNTIGTAMFQ ALRVGLNCAG FSKEYASANS
     351  ELVNEQIRTW ICCNVVSQTV ASSFGFPAYV SFDYLVISSI RVPNSKSQVD
     401  IPNELRQMAQ IARFENQIVN TMNSTPASVT GMVSQEEKQP LLHVLNQQLS
     451  QLEISLEENN LDDIRKFLLL VAKVHLLTYY FTDVTSQSAG KSNGNIYEGS
     501  YSIMELDTSF ETKRGLVKVY NAAVNFLIHA NSMWEHDPTI IKYFPGLFVL
     551  NIWQSACIIS KLIHSSLHSM LDVNSGKKAY NNAISLTFNA SVLKYDMAYR
     601  SSGIMRSIWS LFANMYDAWK NDQKEGGGRL NNDFNLGITI KSRMSVNVFF
     651  DCLYILKEKC GMAKLERETK VSTAYNVDEE EEEDEDEEGE EEEEEEELSS
     701  KVPENMDSQQ LRTRKFTNVR HPEKKARKII ETIPLDPNPI NAGSTSSGSS
     751  LTTPNSQVAN TISYRGILNK MSPREQLNHA NLDSSVSTDI KDTEAVNEPL
     801  PIGRNAEHPA NQPPLSITQM QENTLPATQA NSSLLETYPI VQSNPVTTTI
     851  KESPNSIMAG WDNWESDMVW RDVDILMNEF AFNPKV*

external links for Leu3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Leu3p

1)	Friden P and Schimmel P (1988) LEU3 of Saccharomyces cerevisiae activates multiple genes for branched-chain amino acid biosynthesis by binding to a common decanucleotide core sequence. Mol Cell Biol 8(7):2690-7
2)	Zhou K, et al. (1987) Structure of yeast regulatory gene LEU3 and evidence that LEU3 itself is under general amino acid control. Nucleic Acids Res 15(13):5261-73
3)	Zhou KM, et al. (1990) Yeast regulatory protein LEU3: a structure-function analysis. Nucleic Acids Res 18(2):291-8
4)	Friden P and Schimmel P (1987) LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes. Mol Cell Biol 7(8):2708-17
5)	Sze JY, et al. (1992) In vitro transcriptional activation by a metabolic intermediate: activation by Leu3 depends on alpha-isopropylmalate. Science 258(5085):1143-5
6)	Beltzer JP, et al. (1988) Yeast LEU4 encodes mitochondrial and nonmitochondrial forms of alpha-isopropylmalate synthase. J Biol Chem 263(1):368-74
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41