SEN1/YLR430W Protein Information 
| Standard Name | Sen1p 1 |
|---|---|
| Systematic Name | Ylr430wp |
| Alias | Cik3p , Nrd2p |
| ORF Classification | Verified |
| Description | Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS (2, 3, 4, 5, 6, 7) |
| Name Description | Splicing ENdonuclease 1 |
| Experimental Data | |
|---|---|
| Molecules/cell | 125 8 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Sen1p (InterPro) |
| Physical Interactions | There are 78 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI0000135849 | Q00416 MIPS: YLR430W NCBI: 172574 | 2340994 | 3123282 | 398366241 | 664872 | NP_013534.3 | NM_001182318.3 GenBank/EMBL/DDBJ: DAA09731.1 | M74589 | U20939 | U21094 |
| External Classifications | EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.- |
external links for Sen1p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | YPL+ |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YeastGFP |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastRC Public Image Repository |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | |
| Candida (CandidaDB) | Complexome | YeastRC Structure Prediction (Seattle) | |
| YGOB | DIP | ||
| YOGY | GeneMANIA | ||
| YeastRC Two-Hybrid (Seattle) |
References cited on this page View Complete Literature Guide for Sen1p
| 1) | Winey M and Culbertson MR (1988) Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae. Genetics 118(4):609-17 |
| 2) | Ursic D, et al. (1997) The yeast SEN1 gene is required for the processing of diverse RNA classes. Nucleic Acids Res 25(23):4778-85 |
| 3) | Rasmussen TP and Culbertson MR (1998) The putative nucleic acid helicase Sen1p is required for formation and stability of termini and for maximal rates of synthesis and levels of accumulation of small nucleolar RNAs in Saccharomyces cerevisiae. Mol Cell Biol 18(12):6885-96 |
| 4) | Steinmetz EJ, et al. (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31 |
| 5) | Chen YZ, et al. (2006) Senataxin, the yeast Sen1p orthologue: characterization of a unique protein in which recessive mutations cause ataxia and dominant mutations cause motor neuron disease. Neurobiol Dis 23(1):97-108 |
| 6) | Steinmetz EJ, et al. (2006) Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase. Mol Cell 24(5):735-46 |
| 7) | Alzu A, et al. (2012) Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell 151(4):835-46 |
| 8) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
