RPN13/YLR421C Protein Information Help

Standard Name Rpn13p 1
Systematic Name Ylr421cp
ORF Classification Verified
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description Regulatory Particle Non-ATPase 5
Experimental Data
Molecules/cell 12200 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 156
Molecular Weight (Da) 17,902
Isoelectric Point (pI) 4.28

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rpn13p (InterPro)
Physical Interactions There are 88 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000531A7 | O13563
MIPS: YLR421C
NCBI: 188595916 | 2340992 | 398366191 | 403071984 | 45270532 | 74644735 | NP_013525.3
GenBank/EMBL/DDBJ: DAA09723.1 | AY558321 | U20939
Amino Acid Sequence (or in FASTA format)
 
       1  MSMSSTVIKF RAGVCEYNED SRLCTPIPVQ GEIEIKPNEE EELGFWDFEW
      51  RPTEKPVGRE LDPISLILIP GETMWVPIKS SKSGRIFALV FSSNERYFFW
     101  LQEKNSGNLP LNELSAKDKE IYNKMIGVLN NSSESDEEES NDEKQKAQDV
     151  DVSMQD*

external links for Rpn13p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome


DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Rpn13p
1) Verma R, et al.  (2000) Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol Biol Cell 11(10):3425-39
2) Seong KM, et al.  (2007) Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. FEBS Lett 581(13):2567-73
3) Husnjak K, et al.  (2008) Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 453(7194):481-488
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41