SFP1/YLR403W Protein Information Help

Standard Name Sfp1p 1
Systematic Name Ylr403wp
ORF Classification Verified
Description Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion (10, 11, 2, 3, 4, 5, 6, 7, 8, 9) Also known as: [ISP(+)] 12 , [ISP+]
Name Description Split Finger Protein 1
Experimental Data
Molecules/cell 259 13
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 683
Molecular Weight (Da) 74,785
Isoelectric Point (pI) 6.69

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sfp1p (InterPro)
Physical Interactions There are 34 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001358B6 | P32432
MIPS: YLR403W
NCBI: 172591 | 417770 | 625101 | 6323435 | NP_013507.1 | NM_001182291.1
GenBank/EMBL/DDBJ: AAA35041.1 | AAB82343.1 | DAA09702.1 | M63577 | U19729
Amino Acid Sequence (or in FASTA format)
       1  MDFTTMTMAS NMATSTTTTA TSAHASINSS SNFNIDIDSN QNTPSILINN
      51  NSDSSNGKNT DFNGVNNIHQ KNIMNNTNNV HLYSPNIMDQ TLLTPQDIAK
     101  LRRESIAHSQ GMGGVSWGSI SVGSWLRDEI ISRRNSIVPA SANGAASAAA
     151  SATTTATNTL QIQQPTKRPS VSNPPYHRGY SISPQIAYTA YLPNLEKQYC
     201  KDYSCCGLSL PGLHDLLRHY EEAHISTSPN TTNMSQIPMN SAGNTSSSVR
     251  MTNNTSSANY NLQNNMAANT KNAGHKTNTM QAHSSNATNN TSINNMHANL
     301  QSNMDSNSTI RQSQHPHHQQ NIIQQQLQSN SVNHTSGAVP TPSVMGSATA
     351  SSTTANPNVI SITGAPNSGL SMANHSQQLH LNGNLVDAVS TNDVFLRTSN
     401  SPSRHVPHNK QINSNNNSGI NINNNTSHNS NINMGSKNAM VNRPHTFNNY
     451  SLNKTSRNPI QHQSRKIDPH QTDLSPLVLV QDIDLSFMDD DILGPSNHNS
     501  MNSVVNPTTG SHNYNTFHSS VHAKSSQNMV EDQDIDDIDD DDDVDDDDDD
     551  DDDDDTENGS SSNGKSVHNN NYKMPQQAYI DDPARRLYVM DHEEQKPFKC
     601  PVIGCEKTYK NQNGLKYHRL HGHQNQKLHE NPDGTFSVID PDSTDSFGDG
     651  MGSAKDKPYR CEVCGKRYKN LNGLKYHRGH STH*                 

external links for Sfp1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains YeastRC Public Image Repository
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Sfp1p
1) Blumberg H and Silver P  (1991) A split zinc-finger protein is required for normal yeast growth. Gene 107(1):101-10
2) Xu Z and Norris D  (1998) The SFP1 gene product of Saccharomyces cerevisiae regulates G2/M transitions during the mitotic cell cycle and DNA-damage response. Genetics 150(4):1419-28
3) Bohm S, et al.  (1997) Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins. Nucleic Acids Res 25(12):2464-9
4) Jorgensen P, et al.  (2002) Systematic identification of pathways that couple cell growth and division in yeast. Science 297(5580):395-400
5) Jorgensen P, et al.  (2004) A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size. Genes Dev 18(20):2491-505
6) Marion RM, et al.  (2004) Sfp1 is a stress- and nutrient-sensitive regulator of ribosomal protein gene expression. Proc Natl Acad Sci U S A 101(40):14315-22
7) Cipollina C, et al.  (2005) SFP1 is involved in cell size modulation in respiro-fermentative growth conditions. Yeast 22(5):385-99
8) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
9) Lempiainen H, et al.  (2009) Sfp1 interaction with TORC1 and Mrs6 reveals feedback regulation on TOR signaling. Mol Cell 33(6):704-16
10) Gordan R, et al.  (2009) Distinguishing direct versus indirect transcription factor-DNA interactions. Genome Res 19(11):2090-100
11) Zeevi D, et al.  (2011) Compensation for differences in gene copy number among yeast ribosomal proteins is encoded within their promoters. Genome Res 21(12):2114-28
12) Volkov KV, et al.  (2002) Novel non-Mendelian determinant involved in the control of translation accuracy in Saccharomyces cerevisiae. Genetics 160(1):25-36
13) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41