DUS3/YLR401C Protein Information Help

Standard Name Dus3p 1
Systematic Name Ylr401cp
ORF Classification Verified
Description Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress (2, 3, 4)
Name Description DihydroUridine Synthase 1, 3
Experimental Data
Molecules/cell 3630 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 668
Molecular Weight (Da) 76,595
Isoelectric Point (pI) 7.38

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dus3p (InterPro)
Physical Interactions There are 7 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00006842A8 | Q06053
MIPS: YLR401C
NCBI: 187608869 | 2833203 | 398366123 | 547573 | 625116 | NP_013505.4
GenBank/EMBL/DDBJ: DAA09701.1 | U19729 | Z18944
External Classifications EC: 1.-.-.- [Oxidoreductases]
EC: 1.3.1.89 [tRNA-dihydrouridine(47) synthase (NAD(P)(+))]
Amino Acid Sequence (or in FASTA format)
 
       1  MEQNAEKRSI VGDDNSTVKR QDTSPSKGIA HIKPEYIVPL KQNENQKVAI
      51  YDEEMSSDRM TNEFAGGTNK KNKNGRGKKR GQNKNRDNRQ VKEQNVLCPR
     101  LIHGDISKCS FGDNCRFVHD INLYLSTKKP EVESNIFPSC PVFNSLGFCP
     151  MGFKCRFLSS HLNKEDNILI SKKEIDPDAQ TIWSVKGEVN HISPERKLDL
     201  IKRRFPFTKS NEILEIIDSF QQECRDSMKP EEEVESTPQL KKQDPDVEQP
     251  VAPQVEQRNK ELSEHRMKQR EVYLKYKDTR YFAQEKKPLD LYHKKIVSPL
     301  TTVGNLPYRR LMRKLGADVT YSEMALAVPL IQGTNSEWAL PKAHTSEFPG
     351  FGVQVACSKA WQAAKAAEAL ANSVSEISEI NLNSGCPIDL LYRQGSGSAL
     401  LDNPARMIRC LNAMNYVSKD IPITVKIRTG TKEGHPIAEG LVKRLVNETD
     451  VAAITLHGRS RQQRYTKSAD WDYVSQVADT LRSAEADFIE TEQGKEGRDS
     501  KNRIQFVGNG DVNNFEDWYR YLNGNENIDS VMVARGALIK PWIFEEVESQ
     551  QYLDKTSTER LDILRDYAQF SMEHWGTDEY GISQCRRFFC EFMSFFHRYV
     601  PMGICERYPV KLNERPPNWC GRDELETLMG STDVNDWIKL SDLFFGKTDE
     651  NFVFVPKHKS SSYANRDS*

external links for Dus3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome

YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Dus3p
1) Xing F, et al.  (2004) The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs. J Biol Chem 279(17):17850-60
2) Karpichev IV and Small GM  (1998) Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae. Mol Cell Biol 18(11):6560-70
3) Xing F, et al.  (2002) A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA. RNA 8(3):370-81
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41