SKI2/YLR398C Protein Information

Standard Name	Ski2p 1
Systematic Name	Ylr398cp
ORF Classification	Verified
Description	Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome (1, 2, 3, 4, 5)
Name Description	SuperKIller 1

Experimental Data
Molecules/cell	5770 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,287
Molecular Weight (Da)	146,057
Isoelectric Point (pI)	6.72

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ski2p (InterPro)
Physical Interactions	There are 49 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005318A \| P35207 MIPS: YLR398C NCBI: 364506251 \| 364506252 \| 364506253 \| 364506254 \| 364506255 \| 364506256 \| 398366119 \| 410320 \| 547574 \| 625114 \| 8489004 \| NP_013502.3 \| NM_001182286.3 GenBank/EMBL/DDBJ: DAA09699.1 \| L13469 \| U19729 \| Z18944
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.13 [RNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MSEGFSSSSI QELYQSLKEI TNNADVELFE DRITKLDFES TDEPKHANDI 51 IKDRFLRPSN ALPWSLLDMV QDVPHTSSPE DCSGKLDYKE LLKVPDPINR 101 TSYQFKRTGL EGKISGYKEE VDLKEVANAN ASNSLSITRS INHNQNSVRG 151 STAQLPFTPG GIPMKSVKTD SEQNGSSTMA NATKLLHKDG QGLFDIPEGM 201 NRGIKPMDSP AENEDQNGQF KELKQLNEID NELDIRIEAN EAKLKEEEKS 251 AKSISEEIME EATEETTADN ADDAEIDELL PIGIDFGRTK PVSKSVPVKK 301 EWAHVVDLNH KIENFDELIP NPARSWPFEL DTFQKEAVYH LEQGDSVFVA 351 AHTSAGKTVV AEYAIAMAHR NMTKTIYTSP IKALSNQKFR DFKETFDDVN 401 IGLITGDVQI NPDANCLIMT TEILRSMLYR GADLIRDVEF VIFDEVHYVN 451 DQDRGVVWEE VIIMLPQHVK FILLSATVPN TYEFANWIGR TKQKNIYVIS 501 TPKRPVPLEI NIWAKKELIP VINQNSEFLE ANFRKHKEIL NGESAKGAPS 551 KTDNGRGGST ARGGRGGSNT RDGRGGRGNS TRGGANRGGS RGAGAIGSNK 601 RKFFTQDGPS KKTWPEIVNY LRKRELLPMV VFVFSKKRCE EYADWLEGIN 651 FCNNKEKSQI HMFIEKSITR LKKEDRDLPQ ILKTRSLLER GIAVHHGGLL 701 PIVKELIEIL FSKGFIKVLF ATETFAMGLN LPTRTVIFSS IRKHDGNGLR 751 ELTPGEFTQM AGRAGRRGLD STGTVIVMAY NSPLSIATFK EVTMGVPTRL 801 QSQFRLTYNM ILNLLRIEAL RVEEMIKYSF SENAKETLQP EHEKQIKVLQ 851 EELQTIEYKS CEICDNDIEK FLELMLAYKE ATVNLMQEMV KSPSILHILK 901 EGRLVAFRDP NDCLKLGFVF KVSLKDAVCV IMTFTKPYKL PNGEPNHLIY 951 FPKADGYRRR NFPKFQKTDF YMEEVPVTAI EVITKRKFAA PLGKVIKKDV 1001 AALNEFNAET NNILDGKTLK EAINIEKQGL KIHQILLDRT NIRDEIFKLK 1051 SIKCPNLSQH IVPKFKAHVI KKKIEELYHL MSDQNLSLLP DYEKRLAVLK 1101 DTEFIDQNHN VLLKGRVACE INSGYELVLT ELILDNFLGS FEPEEIVALL 1151 SVFVYEGKTR EEEPPIVTPR LAKGKQRIEE IYKKMLCVFN THQIPLTQDE 1201 AEFLDRKRFA MMNVVYEWAR GLSFKEIMEM SPEAEGTVVR VITWLDEICR 1251 EVKTASIIIG NSTLHMKMSR AQELIKRDIV FAASLYL*

Amino Acid Sequence (or in FASTA format)

       1  MSEGFSSSSI QELYQSLKEI TNNADVELFE DRITKLDFES TDEPKHANDI
      51  IKDRFLRPSN ALPWSLLDMV QDVPHTSSPE DCSGKLDYKE LLKVPDPINR
     101  TSYQFKRTGL EGKISGYKEE VDLKEVANAN ASNSLSITRS INHNQNSVRG
     151  STAQLPFTPG GIPMKSVKTD SEQNGSSTMA NATKLLHKDG QGLFDIPEGM
     201  NRGIKPMDSP AENEDQNGQF KELKQLNEID NELDIRIEAN EAKLKEEEKS
     251  AKSISEEIME EATEETTADN ADDAEIDELL PIGIDFGRTK PVSKSVPVKK
     301  EWAHVVDLNH KIENFDELIP NPARSWPFEL DTFQKEAVYH LEQGDSVFVA
     351  AHTSAGKTVV AEYAIAMAHR NMTKTIYTSP IKALSNQKFR DFKETFDDVN
     401  IGLITGDVQI NPDANCLIMT TEILRSMLYR GADLIRDVEF VIFDEVHYVN
     451  DQDRGVVWEE VIIMLPQHVK FILLSATVPN TYEFANWIGR TKQKNIYVIS
     501  TPKRPVPLEI NIWAKKELIP VINQNSEFLE ANFRKHKEIL NGESAKGAPS
     551  KTDNGRGGST ARGGRGGSNT RDGRGGRGNS TRGGANRGGS RGAGAIGSNK
     601  RKFFTQDGPS KKTWPEIVNY LRKRELLPMV VFVFSKKRCE EYADWLEGIN
     651  FCNNKEKSQI HMFIEKSITR LKKEDRDLPQ ILKTRSLLER GIAVHHGGLL
     701  PIVKELIEIL FSKGFIKVLF ATETFAMGLN LPTRTVIFSS IRKHDGNGLR
     751  ELTPGEFTQM AGRAGRRGLD STGTVIVMAY NSPLSIATFK EVTMGVPTRL
     801  QSQFRLTYNM ILNLLRIEAL RVEEMIKYSF SENAKETLQP EHEKQIKVLQ
     851  EELQTIEYKS CEICDNDIEK FLELMLAYKE ATVNLMQEMV KSPSILHILK
     901  EGRLVAFRDP NDCLKLGFVF KVSLKDAVCV IMTFTKPYKL PNGEPNHLIY
     951  FPKADGYRRR NFPKFQKTDF YMEEVPVTAI EVITKRKFAA PLGKVIKKDV
    1001  AALNEFNAET NNILDGKTLK EAINIEKQGL KIHQILLDRT NIRDEIFKLK
    1051  SIKCPNLSQH IVPKFKAHVI KKKIEELYHL MSDQNLSLLP DYEKRLAVLK
    1101  DTEFIDQNHN VLLKGRVACE INSGYELVLT ELILDNFLGS FEPEEIVALL
    1151  SVFVYEGKTR EEEPPIVTPR LAKGKQRIEE IYKKMLCVFN THQIPLTQDE
    1201  AEFLDRKRFA MMNVVYEWAR GLSFKEIMEM SPEAEGTVVR VITWLDEICR
    1251  EVKTASIIIG NSTLHMKMSR AQELIKRDIV FAASLYL*

external links for Ski2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ski2p

1)	Toh-E A, et al. (1978) Chromosomal superkiller mutants of Saccharomyces cerevisiae. J Bacteriol 136(3):1002-7
2)	Brown JT, et al. (2000) The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo. RNA 6(3):449-57
3)	Widner WR and Wickner RB (1993) Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA. Mol Cell Biol 13(7):4331-41
4)	Anderson JS and Parker RP (1998) The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex. EMBO J 17(5):1497-506
5)	Fabre A, et al. (2012) SKIV2L mutations cause syndromic diarrhea, or trichohepatoenteric syndrome. Am J Hum Genet 90(4):689-92
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41