AFG2/YLR397C Protein Information Help

Standard Name Afg2p
Systematic Name Ylr397cp
Alias Drg1p
ORF Classification Verified
Description ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs (1, 2, 3, 4)
Name Description ATPase Family Gene 1
Experimental Data
Molecules/cell 799 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 780
Molecular Weight (Da) 84,747
Isoelectric Point (pI) 5.5

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Afg2p (InterPro)
Physical Interactions There are 23 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005328C | P32794
MIPS: YLR397C
NCBI: 295573 | 416590 | 51013683 | 625113 | 6323429 | NP_013501.1
GenBank/EMBL/DDBJ: DAA09698.1 | AY693116 | L14615 | U19729
Amino Acid Sequence (or in FASTA format)
 
       1  MAPKSSSSGS KKKSSASSNS ADAKASKFKL PAEFITRPHP SKDHGKETCT
      51  AYIHPNVLSS LEINPGSFCT VGKIGENGIL VIARAGDEEV HPVNVITLST
     101  TIRSVGNLIL GDRLELKKAQ VQPPYATKVT VGSLQGYNIL ECMEEKVIQK
     151  LLDDSGVIMP GMIFQNLKTK AGDESIDVVI TDASDDSLPD VSQLDLNMDD
     201  MYGGLDNLFY LSPPFIFRKG STHITFSKET QANRKYNLPE PLSYAAVGGL
     251  DKEIESLKSA IEIPLHQPTL FSSFGVSPPR GILLHGPPGT GKTMLLRVVA
     301  NTSNAHVLTI NGPSIVSKYL GETEAALRDI FNEARKYQPS IIFIDEIDSI
     351  APNRANDDSG EVESRVVATL LTLMDGMGAA GKVVVIAATN RPNSVDPALR
     401  RPGRFDQEVE IGIPDVDARF DILTKQFSRM SSDRHVLDSE AIKYIASKTH
     451  GYVGADLTAL CRESVMKTIQ RGLGTDANID KFSLKVTLKD VESAMVDIRP
     501  SAMREIFLEM PKVYWSDIGG QEELKTKMKE MIQLPLEASE TFARLGISAP
     551  KGVLLYGPPG CSKTLTAKAL ATESGINFLA VKGPEIFNKY VGESERAIRE
     601  IFRKARSAAP SIIFFDEIDA LSPDRDGSST SAANHVLTSL LNEIDGVEEL
     651  KGVVIVAATN RPDEIDAALL RPGRLDRHIY VGPPDVNARL EILKKCTKKF
     701  NTEESGVDLH ELADRTEGYS GAEVVLLCQE AGLAAIMEDL DVAKVELRHF
     751  EKAFKGIARG ITPEMLSYYE EFALRSGSSS *

external links for Afg2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Afg2p
1) Thorsness PE, et al.  (1993) AFG2, an essential gene in yeast, encodes a new member of the Sec18p, Pas1p, Cdc48p, TBP-1 family of putative ATPases. Yeast 9(11):1267-71
2) Zakalskiy A, et al.  (2002) Structural and enzymatic properties of the AAA protein Drg1p from Saccharomyces cerevisiae. Decoupling of intracellular function from ATPase activity and hexamerization. J Biol Chem 277(30):26788-95
3) Pertschy B, et al.  (2007) Cytoplasmic Recycling of 60S Preribosomal Factors Depends on the AAA Protein Drg1. Mol Cell Biol 27(19):6581-92
4) Kappel L, et al.  (2012) Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation. J Cell Biol 199(5):771-82
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41