VPS33/YLR396C Protein Information Help

Standard Name Vps33p 1, 2
Systematic Name Ylr396cp
Alias Cls14p 3 , Met27p 4 , Pep14p , Slp1p 5, 6 , Vam5p 7 , Vpl25p 8 , Vpt33p 1
ORF Classification Verified
Description ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole (10, 11, 12, 9)
Name Description Vacuolar Protein Sorting 1, 2
Experimental Data
Molecules/cell 830 13
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 691
Molecular Weight (Da) 79,271
Isoelectric Point (pI) 5.02

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Vps33p (InterPro)
Physical Interactions There are 131 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052FFE | P20795
MIPS: YLR396C
NCBI: 134528 | 172619 | 173185 | 398366111 | 625112 | NP_013500.3 | NM_001182284.3
GenBank/EMBL/DDBJ: DAA09697.1 | M34474 | M34638 | U19729
Amino Acid Sequence (or in FASTA format)
 
       1  MNRFWNTKKF SLTNADGLCA TLNEISQNDE VLVVQPSVLP VLNSLLTFQD
      51  LTQSTPVRKI TLLDDQLSDD LPSALGSVPQ MDLIFLIDVR TSLRLPPQLL
     101  DAAQKHNLSS LHIIYCRWKP SFQNTLEDTE QWQKDGFDLN SKKTHFPNVI
     151  ESQLKELSNE YTLYPWDLLP FPQIDENVLL THSLYNMENV NMYYPNLRSL
     201  QSATESILVD DMVNSLQSLI FETNSIITNV VSIGNLSKRC SHLLKKRIDE
     251  HQTENDLFIK GTLYGERTNC GLEMDLIILE RNTDPITPLL TQLTYAGILD
     301  DLYEFNSGIK IKEKDMNFNY KEDKIWNDLK FLNFGSIGPQ LNKLAKELQT
     351  QYDTRHKAES VHEIKEFVDS LGSLQQRQAF LKNHTTLSSD VLKVVETEEY
     401  GSFNKILELE LEILMGNTLN NDIEDIILEL QYQYEVDQKK ILRLICLLSL
     451  CKNSLREKDY EYLRTFMIDS WGIEKCFQLE SLAELGFFTS KTGKTDLHIT
     501  TSKSTRLQKE YRYISQWFNT VPIEDEHAAD KITNENDDFS EATFAYSGVV
     551  PLTMRLVQML YDRSILFHNY SSQQPFILSR EPRVSQTEDL IEQLYGDSHA
     601  IEESIWVPGT ITKKINASIK SNNRRSIDGS NGTFHAAEDI ALVVFLGGVT
     651  MGEIAIMKHL QKILGKKGIN KRFIIIADGL INGTRIMNSI S*

external links for Vps33p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Vps33p
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Ohya Y, et al.  (1986) Isolation and characterization of Ca2+-sensitive mutants of Saccharomyces cerevisiae. J Gen Microbiol 132(4):979-88
4) Jacquemin-Faure I, et al.  (1994) The vacuolar compartment is required for sulfur amino acid homeostasis in Saccharomyces cerevisiae. Mol Gen Genet 244(5):519-29
5) Kitamoto K, et al.  (1988) Mutants of Saccharomyces cerevisiae with defective vacuolar function. J Bacteriol 170(6):2687-91
6) Wada Y, et al.  (1990) The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function. Mol Cell Biol 10(5):2214-23
7) Wada Y, et al.  (1992) Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. I. Isolation and characterization of two classes of vam mutants. J Biol Chem 267(26):18665-70
8) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
9) Seals DF, et al.  (2000) A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion. Proc Natl Acad Sci U S A 97(17):9402-7
10) Gerhardt B, et al.  (1998) The vesicle transport protein Vps33p is an ATP-binding protein that localizes to the cytosol in an energy-dependent manner. J Biol Chem 273(25):15818-29
11) Darsow T, et al.  (1997) A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole. J Cell Biol 138(3):517-29
12) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
13) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41