NAM2/YLR382C Protein Information

Standard Name	Nam2p 1
Systematic Name	Ylr382cp
Alias	Msl1p
ORF Classification	Verified
Description	Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance (2, 3, 4, 5)
Name Description	Nuclear Accommodation of Mitochondria 1
Gene Product	LeuRS 6 , mitochondrial leucyl-tRNA synthetase 6

Experimental Data
Molecules/cell	2120 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	894
Molecular Weight (Da)	101,920
Isoelectric Point (pI)	8.48

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nam2p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136554 \| P11325 MIPS: YLR382C NCBI: 135144 \| 172008 \| 398366053 \| 4021 \| 609429 \| NP_013486.3 \| NM_001182271.3 GenBank/EMBL/DDBJ: DAA09683.1 \| J03495 \| U19104 \| X05143
External Classifications	EC: 6.1.1.4 [Leucine--tRNA ligase]

Amino Acid Sequence (or in FASTA format)
1 MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY 51 ILCQFPYPSG ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA 101 ENAAIERSIN PAIWTRDNIA KMKQQMQSML ANFDWDREIT TCDPEYYKFT 151 QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL ANEQVDAQGR SWRSGAIVEK 201 KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG ESVGAELVFK 251 VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 301 SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC 351 PGHDNRDFEF WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS 401 TDGVLTKECG EHSGVLTVVA RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ 451 RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELEGLDTKGN PLSTIDEFVN 501 VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASKNMP 551 VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 601 QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS 651 KYNGADPNEC ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV 701 LHLTKNILSL EKDLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV 751 NLSLNTVISD YMKLTNILES ALKKGEVRNE MIVQNLQKLV TVIYPAVPSI 801 SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKN 851 FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK*

Amino Acid Sequence (or in FASTA format)

       1  MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY
      51  ILCQFPYPSG ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA
     101  ENAAIERSIN PAIWTRDNIA KMKQQMQSML ANFDWDREIT TCDPEYYKFT
     151  QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL ANEQVDAQGR SWRSGAIVEK
     201  KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG ESVGAELVFK
     251  VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK
     301  SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC
     351  PGHDNRDFEF WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS
     401  TDGVLTKECG EHSGVLTVVA RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ
     451  RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELEGLDTKGN PLSTIDEFVN
     501  VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASKNMP
     551  VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV
     601  QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS
     651  KYNGADPNEC ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV
     701  LHLTKNILSL EKDLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV
     751  NLSLNTVISD YMKLTNILES ALKKGEVRNE MIVQNLQKLV TVIYPAVPSI
     801  SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKN
     851  FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK*

external links for Nam2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Nam2p

1)	Dujardin G, et al. (1980) Long range control circuits within mitochondria and between nucleus and mitochondria. I. Methodology and phenomenology of suppressors. Mol Gen Genet 179(3):469-82
2)	Labouesse M (1990) The yeast mitochondrial leucyl-tRNA synthetase is a splicing factor for the excision of several group I introns. Mol Gen Genet 224(2):209-21
3)	Zagorski W, et al. (1991) Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase. J Biol Chem 266(4):2537-41
4)	Labouesse M, et al. (1985) The yeast nuclear gene NAM2 is essential for mitochondrial DNA integrity and can cure a mitochondrial RNA-maturase deficiency. Cell 41(1):133-43
5)	Rho SB and Martinis SA (2000) The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. RNA 6(12):1882-94
6)	Herbert CJ, et al. (1988) The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing. EMBO J 7(2):473-83
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41