ILV5/YLR355C Protein Information

Standard Name	Ilv5p
Systematic Name	Ylr355cp
ORF Classification	Verified
Description	Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids (1, 2, 3, 4)
Name Description	IsoLeucine-plus-Valine requiring

Experimental Data
Molecules/cell	883000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	395
Molecular Weight (Da)	44,368
Isoelectric Point (pI)	9.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ilv5p (InterPro)
Physical Interactions	There are 33 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012D529 \| P06168 MIPS: YLR355C NCBI: 124371 \| 3827 \| 609403 \| 6323387 \| NP_013459.1 GenBank/EMBL/DDBJ: DAA09659.1 \| U19102 \| X04969
External Classifications	EC: 1.1.1.86 [Ketol-acid reductoisomerase]

Amino Acid Sequence (or in FASTA format)
1 MLRTQAARLI CNSRVITAKR TFALATRAAA YSRPAARFVK PMITTRGLKQ 51 INFGGTVETV YERADWPREK LLDYFKNDTF ALIGYGSQGY GQGLNLRDNG 101 LNVIIGVRKD GASWKAAIED GWVPGKNLFT VEDAIKRGSY VMNLLSDAAQ 151 SETWPAIKPL LTKGKTLYFS HGFSPVFKDL THVEPPKDLD VILVAPKGSG 201 RTVRSLFKEG RGINSSYAVW NDVTGKAHEK AQALAVAIGS GYVYQTTFER 251 EVNSDLYGER GCLMGGIHGM FLAQYDVLRE NGHSPSEAFN ETVEEATQSL 301 YPLIGKYGMD YMYDACSTTA RRGALDWYPI FKNALKPVFQ DLYESTKNGT 351 ETKRSLEFNS QPDYREKLEK ELDTIRNMEI WKVGKEVRKL RPENQ*

Amino Acid Sequence (or in FASTA format)

       1  MLRTQAARLI CNSRVITAKR TFALATRAAA YSRPAARFVK PMITTRGLKQ
      51  INFGGTVETV YERADWPREK LLDYFKNDTF ALIGYGSQGY GQGLNLRDNG
     101  LNVIIGVRKD GASWKAAIED GWVPGKNLFT VEDAIKRGSY VMNLLSDAAQ
     151  SETWPAIKPL LTKGKTLYFS HGFSPVFKDL THVEPPKDLD VILVAPKGSG
     201  RTVRSLFKEG RGINSSYAVW NDVTGKAHEK AQALAVAIGS GYVYQTTFER
     251  EVNSDLYGER GCLMGGIHGM FLAQYDVLRE NGHSPSEAFN ETVEEATQSL
     301  YPLIGKYGMD YMYDACSTTA RRGALDWYPI FKNALKPVFQ DLYESTKNGT
     351  ETKRSLEFNS QPDYREKLEK ELDTIRNMEI WKVGKEVRKL RPENQ*

external links for Ilv5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ilv5p

1)	Petersen JG and Holmberg S (1986) The ILV5 gene of Saccharomyces cerevisiae is highly expressed. Nucleic Acids Res 14(24):9631-51
2)	Zelenaya-Troitskaya O, et al. (1995) An enzyme in yeast mitochondria that catalyzes a step in branched-chain amino acid biosynthesis also functions in mitochondrial DNA stability. EMBO J 14(13):3268-76
3)	Park S, et al. (2006) Yme2p is a mediator of nucleoid structure and number in mitochondria of the yeast Saccharomyces cerevisiae. Curr Genet 50(3):173-82
4)	Macierzanka M, et al. (2008) Maintenance and stabilization of mtDNA can be facilitated by the DNA-binding activity of Ilv5p. Biochim Biophys Acta 1783(1):107-17
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41