FKS1/YLR342W Protein Information

Standard Name	Fks1p
Systematic Name	Ylr342wp
Alias	Cnd1p 1 , Cwh53p , Etg1p , Gsc1p , Pbr1p
ORF Classification	Verified
Description	Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	FK506 Sensitivity 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,876
Molecular Weight (Da)	214,849
Isoelectric Point (pI)	7.19

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fks1p (InterPro)
Transmembrane Domains	There are 13 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 72 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E1D \| P38631 MIPS: YLR342W NCBI: 1346146 \| 40457585 \| 40457587 \| 40457589 \| 532663 \| 562002 \| 563150 \| 577608 \| 577657 \| 609385 \| 6323374 \| 683791 \| NP_013446.1 \| NM_001182231.1 GenBank/EMBL/DDBJ: DAA09646.1 \| AY395693 \| AY395694 \| AY395695 \| D42126 \| L35923 \| U08459 \| U12893 \| U19028 \| X80817 \| Z46262
External Classifications	EC: 2.4.1.34 [1,3-beta-glucan synthase]

Amino Acid Sequence (or in FASTA format)
1 MNTDQQPYQG QTDYTQGPGN GQSQEQDYDQ YGQPLYPSQA DGYYDPNVAA 51 GTEADMYGQQ PPNESYDQDY TNGEYYGQPP NMAAQDGENF SDFSSYGPPG 101 TPGYDSYGGQ YTASQMSYGE PNSSGTSTPI YGNYDPNAIA MALPNEPYPA 151 WTADSQSPVS IEQIEDIFID LTNRLGFQRD SMRNMFDHFM VLLDSRSSRM 201 SPDQALLSLH ADYIGGDTAN YKKWYFAAQL DMDDEIGFRN MSLGKLSRKA 251 RKAKKKNKKA MEEANPEDTE ETLNKIEGDN SLEAADFRWK AKMNQLSPLE 301 RVRHIALYLL CWGEANQVRF TAECLCFIYK CALDYLDSPL CQQRQEPMPE 351 GDFLNRVITP IYHFIRNQVY EIVDGRFVKR ERDHNKIVGY DDLNQLFWYP 401 EGIAKIVLED GTKLIELPLE ERYLRLGDVV WDDVFFKTYK ETRTWLHLVT 451 NFNRIWVMHI SIFWMYFAYN SPTFYTHNYQ QLVDNQPLAA YKWASCALGG 501 TVASLIQIVA TLCEWSFVPR KWAGAQHLSR RFWFLCIIFG INLGPIIFVF 551 AYDKDTVYST AAHVVAAVMF FVAVATIIFF SIMPLGGLFT SYMKKSTRRY 601 VASQTFTAAF APLHGLDRWM SYLVWVTVFA AKYSESYYFL VLSLRDPIRI 651 LSTTAMRCTG EYWWGAVLCK VQPKIVLGLV IATDFILFFL DTYLWYIIVN 701 TIFSVGKSFY LGISILTPWR NIFTRLPKRI YSKILATTDM EIKYKPKVLI 751 SQVWNAIIIS MYREHLLAID HVQKLLYHQV PSEIEGKRTL RAPTFFVSQD 801 DNNFETEFFP RDSEAERRIS FFAQSLSTPI PEPLPVDNMP TFTVLTPHYA 851 ERILLSLREI IREDDQFSRV TLLEYLKQLH PVEWECFVKD TKILAEETAA 901 YEGNENEAEK EDALKSQIDD LPFYCIGFKS AAPEYTLRTR IWASLRSQTL 951 YRTISGFMNY SRAIKLLYRV ENPEIVQMFG GNAEGLEREL EKMARRKFKF 1001 LVSMQRLAKF KPHELENAEF LLRAYPDLQI AYLDEEPPLT EGEEPRIYSA 1051 LIDGHCEILD NGRRRPKFRV QLSGNPILGD GKSDNQNHAL IFYRGEYIQL 1101 IDANQDNYLE ECLKIRSVLA EFEELNVEQV NPYAPGLRYE EQTTNHPVAI 1151 VGAREYIFSE NSGVLGDVAA GKEQTFGTLF ARTLSQIGGK LHYGHPDFIN 1201 ATFMTTRGGV SKAQKGLHLN EDIYAGMNAM LRGGRIKHCE YYQCGKGRDL 1251 GFGTILNFTT KIGAGMGEQM LSREYYYLGT QLPVDRFLTF YYAHPGFHLN 1301 NLFIQLSLQM FMLTLVNLSS LAHESIMCIY DRNKPKTDVL VPIGCYNFQP 1351 AVDWVRRYTL SIFIVFWIAF VPIVVQELIE RGLWKATQRF FCHLLSLSPM 1401 FEVFAGQIYS SALLSDLAIG GARYISTGRG FATSRIPFSI LYSRFAGSAI 1451 YMGARSMLML LFGTVAHWQA PLLWFWASLS SLIFAPFVFN PHQFAWEDFF 1501 LDYRDYIRWL SRGNNQYHRN SWIGYVRMSR ARITGFKRKL VGDESEKAAG 1551 DASRAHRTNL IMAEIIPCAI YAAGCFIAFT FINAQTGVKT TDDDRVNSVL 1601 RIIICTLAPI AVNLGVLFFC MGMSCCSGPL FGMCCKKTGS VMAGIAHGVA 1651 VIVHIAFFIV MWVLESFNFV RMLIGVVTCI QCQRLIFHCM TALMLTREFK 1701 NDHANTAFWT GKWYGKGMGY MAWTQPSREL TAKVIELSEF AADFVLGHVI 1751 LICQLPLIII PKIDKFHSIM LFWLKPSRQI RPPIYSLKQT RLRKRMVKKY 1801 CSLYFLVLAI FAGCIIGPAV ASAKIHKHIG DSLDGVVHNL FQPINTTNND 1851 TGSQMSTYQS HYYTHTPSLK TWSTIK*

Amino Acid Sequence (or in FASTA format)

       1  MNTDQQPYQG QTDYTQGPGN GQSQEQDYDQ YGQPLYPSQA DGYYDPNVAA
      51  GTEADMYGQQ PPNESYDQDY TNGEYYGQPP NMAAQDGENF SDFSSYGPPG
     101  TPGYDSYGGQ YTASQMSYGE PNSSGTSTPI YGNYDPNAIA MALPNEPYPA
     151  WTADSQSPVS IEQIEDIFID LTNRLGFQRD SMRNMFDHFM VLLDSRSSRM
     201  SPDQALLSLH ADYIGGDTAN YKKWYFAAQL DMDDEIGFRN MSLGKLSRKA
     251  RKAKKKNKKA MEEANPEDTE ETLNKIEGDN SLEAADFRWK AKMNQLSPLE
     301  RVRHIALYLL CWGEANQVRF TAECLCFIYK CALDYLDSPL CQQRQEPMPE
     351  GDFLNRVITP IYHFIRNQVY EIVDGRFVKR ERDHNKIVGY DDLNQLFWYP
     401  EGIAKIVLED GTKLIELPLE ERYLRLGDVV WDDVFFKTYK ETRTWLHLVT
     451  NFNRIWVMHI SIFWMYFAYN SPTFYTHNYQ QLVDNQPLAA YKWASCALGG
     501  TVASLIQIVA TLCEWSFVPR KWAGAQHLSR RFWFLCIIFG INLGPIIFVF
     551  AYDKDTVYST AAHVVAAVMF FVAVATIIFF SIMPLGGLFT SYMKKSTRRY
     601  VASQTFTAAF APLHGLDRWM SYLVWVTVFA AKYSESYYFL VLSLRDPIRI
     651  LSTTAMRCTG EYWWGAVLCK VQPKIVLGLV IATDFILFFL DTYLWYIIVN
     701  TIFSVGKSFY LGISILTPWR NIFTRLPKRI YSKILATTDM EIKYKPKVLI
     751  SQVWNAIIIS MYREHLLAID HVQKLLYHQV PSEIEGKRTL RAPTFFVSQD
     801  DNNFETEFFP RDSEAERRIS FFAQSLSTPI PEPLPVDNMP TFTVLTPHYA
     851  ERILLSLREI IREDDQFSRV TLLEYLKQLH PVEWECFVKD TKILAEETAA
     901  YEGNENEAEK EDALKSQIDD LPFYCIGFKS AAPEYTLRTR IWASLRSQTL
     951  YRTISGFMNY SRAIKLLYRV ENPEIVQMFG GNAEGLEREL EKMARRKFKF
    1001  LVSMQRLAKF KPHELENAEF LLRAYPDLQI AYLDEEPPLT EGEEPRIYSA
    1051  LIDGHCEILD NGRRRPKFRV QLSGNPILGD GKSDNQNHAL IFYRGEYIQL
    1101  IDANQDNYLE ECLKIRSVLA EFEELNVEQV NPYAPGLRYE EQTTNHPVAI
    1151  VGAREYIFSE NSGVLGDVAA GKEQTFGTLF ARTLSQIGGK LHYGHPDFIN
    1201  ATFMTTRGGV SKAQKGLHLN EDIYAGMNAM LRGGRIKHCE YYQCGKGRDL
    1251  GFGTILNFTT KIGAGMGEQM LSREYYYLGT QLPVDRFLTF YYAHPGFHLN
    1301  NLFIQLSLQM FMLTLVNLSS LAHESIMCIY DRNKPKTDVL VPIGCYNFQP
    1351  AVDWVRRYTL SIFIVFWIAF VPIVVQELIE RGLWKATQRF FCHLLSLSPM
    1401  FEVFAGQIYS SALLSDLAIG GARYISTGRG FATSRIPFSI LYSRFAGSAI
    1451  YMGARSMLML LFGTVAHWQA PLLWFWASLS SLIFAPFVFN PHQFAWEDFF
    1501  LDYRDYIRWL SRGNNQYHRN SWIGYVRMSR ARITGFKRKL VGDESEKAAG
    1551  DASRAHRTNL IMAEIIPCAI YAAGCFIAFT FINAQTGVKT TDDDRVNSVL
    1601  RIIICTLAPI AVNLGVLFFC MGMSCCSGPL FGMCCKKTGS VMAGIAHGVA
    1651  VIVHIAFFIV MWVLESFNFV RMLIGVVTCI QCQRLIFHCM TALMLTREFK
    1701  NDHANTAFWT GKWYGKGMGY MAWTQPSREL TAKVIELSEF AADFVLGHVI
    1751  LICQLPLIII PKIDKFHSIM LFWLKPSRQI RPPIYSLKQT RLRKRMVKKY
    1801  CSLYFLVLAI FAGCIIGPAV ASAKIHKHIG DSLDGVVHNL FQPINTTNND
    1851  TGSQMSTYQS HYYTHTPSLK TWSTIK*

external links for Fks1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Fks1p

1)	Garrett-Engele P, et al. (1995) Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPase. Mol Cell Biol 15(8):4103-14
2)	Inoue SB, et al. (1995) Characterization and gene cloning of 1,3-beta-D-glucan synthase from Saccharomyces cerevisiae. Eur J Biochem 231(3):845-54
3)	Qadota H, et al. (1996) Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 272(5259):279-81
4)	Douglas CM, et al. (1994) The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase. Proc Natl Acad Sci U S A 91(26):12907-11
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Parent SA, et al. (1993) Calcineurin-dependent growth of an FK506- and CsA-hypersensitive mutant of Saccharomyces cerevisiae. J Gen Microbiol 139(12):2973-84