EST2/YLR318W Protein Information Help

Standard Name Est2p 1
Systematic Name Ylr318wp
ORF Classification Verified
Description Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia (2, 3, 4) Also known as: TERT
Name Description Ever Shorter Telomeres 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 884
Molecular Weight (Da) 102,635
Isoelectric Point (pI) 9.68

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Est2p (InterPro)
Physical Interactions There are 14 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0001F7BD58 | Q06163
MIPS: YLR318W
NCBI: 330443678 | 347595722 | 6226218 | 662136 | NP_013422.2
GenBank/EMBL/DDBJ: DAA09627.2 | U20618
External Classifications EC: 2.7.7.49 [RNA-directed DNA polymerase]
Amino Acid Sequence (or in FASTA format)
 
       1  MKILFEFIQD KLDIDLQTNS TYKENLKCGH FNGLDEILTT CFALPNSRKI
      51  ALPCLPGDLS HKAVIDHCII YLLTGELYNN VLTFGYKIAR NEDVNNSLFC
     101  HSANVNVTLL KGAAWKMFHS LVGTYAFVDL LINYTVIQFN GQFFTQIVGN
     151  RCNEPHLPPK WAQRSSSSSA TAAQIKQLTE PVTNKQFLHK LNINSSSFFP
     201  YSKILPSSSS IKKLTDLREA IFPTNLVKIP QRLKVRINLT LQKLLKRHKR
     251  LNYVSILNSI CPPLEGTVLD LSHLSRQSPK ERVLKFIIVI LQKLLPQEMF
     301  GSKKNKGKII KNLNLLLSLP LNGYLPFDSL LKKLRLKDFR WLFISDIWFT
     351  KHNFENLNQL AICFISWLFR QLIPKIIQTF FYCTEISSTV TIVYFRHDTW
     401  NKLITPFIVE YFKTYLVENN VCRNHNSYTL SNFNHSKMRI IPKKSNNEFR
     451  IIAIPCRGAD EEEFTIYKEN HKNAIQPTQK ILEYLRNKRP TSFTKIYSPT
     501  QIADRIKEFK QRLLKKFNNV LPELYFMKFD VKSCYDSIPR MECMRILKDA
     551  LKNENGFFVR SQYFFNTNTG VLKLFNVVNA SRVPKPYELY IDNVRTVHLS
     601  NQDVINVVEM EIFKTALWVE DKCYIREDGL FQGSSLSAPI VDLVYDDLLE
     651  FYSEFKASPS QDTLILKLAD DFLIISTDQQ QVINIKKLAM GGFQKYNAKA
     701  NRDKILAVSS QSDDDTVIQF CAMHIFVKEL EVWKHSSTMN NFHIRSKSSK
     751  GIFRSLIALF NTRISYKTID TNLNSTNTVL MQIDHVVKNI SECYKSAFKD
     801  LSINVTQNMQ FHSFLQRIIE MTVSGCPITK CDPLIEYEVR FTILNGFLES
     851  LSSNTSKFKD NIILLRKEIQ HLQAYIYIYI HIVN*

external links for Est2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Est2p
1) Lendvay TS, et al.  (1996) Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 144(4):1399-412
2) Lingner J, et al.  (1997) Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity. Proc Natl Acad Sci U S A 94(21):11190-5
3) Livengood AJ, et al.  (2002) Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction. Mol Cell Biol 22(7):2366-74
4) Yamaguchi H, et al.  (2005) Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia. N Engl J Med 352(14):1413-24