STT4/YLR305C Protein Information

Standard Name	Stt4p 1
Systematic Name	Ylr305cp
ORF Classification	Verified
Description	Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization (2, 3, 4)
Name Description	STaurosporine and Temperature sensitive 5

Experimental Data
Molecules/cell	846 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,900
Molecular Weight (Da)	214,605
Isoelectric Point (pI)	7.44

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Stt4p (InterPro)
Physical Interactions	There are 22 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E4F \| P37297 MIPS: YLR305C NCBI: 2340969 \| 454207 \| 577220 \| 586048 \| 6323336 \| NP_013408.1 GenBank/EMBL/DDBJ: DAA09614.1 \| D13717 \| U17243 \| U17247
External Classifications	EC: 2.7.1.67 [1-phosphatidylinositol 4-kinase]

Amino Acid Sequence (or in FASTA format)
1 MRFTRGLKAS SSLRAKAIGR LTKLSTGAPN DQNSNGTTLD LITHTLPIFY 51 STNTSKIYTI PLTLSEWEVL TSLCVAIPTT LDLVETMLKE IIAPYFLETP 101 RQRISDVLSS KFKLEQMRNP IELLTFQLTK FMIQACEQYP VLYENIGGII 151 STYFERVLKI FTIKQSGLLS LVGFINAFIQ FPNSTELTKF TWKKLAKLVL 201 RGSFLNEVDK ILNSSATFTN DSIVQYYDAG NELSSAYLLE LISRLQVSLI 251 SHLLNTSHVG ANLSEFLLNQ QYQFYKFDQE VADENDDTKC IDDFFFNVRS 301 NKQFFTDMCK ISLQFCSESH ILDLSTDNRA RFSFDTRAHY LQTLCLIPFI 351 EDTESELFES FTNVVSESID KFFLSDVVTP SLIKAIVASA SLLNFFTEKL 401 SLTLIRMFPL LVASPHITTE TVNDVAKIFT TGLYPLNEDA IVSTIYSMNN 451 LLAVSEDGSP VPVLRERQLT ITSGKNIEKD YFPLRNSSAS LDGTGALLGN 501 TTVGQLSSHD VNSGATMTYH ASLISNCVAA TTTIASYYNT QSITALTISI 551 LTQKVNSMSK ELDGVILNSL ARLAPNTSLT EFSLLLKFFK SRTVIATKID 601 DSALLKNIIK AKCVISKELL ARHFSSDLYF MYLHDLLDSI IASGEVERLE 651 HHRPQTEISR VADQIATYLE PLAALLPVPG DTPLDINKDE VTTNKFRNAW 701 FNFVIHGYHL GGPIVKRNFS FLLTIAYNSP PLASEFPANN KELSLEMNTI 751 LRRGSSNENI KQQKQQITEY FNTNIVQYRT TSSSKIMFLA AAVLLETIRC 801 EAGDCSKTLL YFSDPSILSG SIEKCIAVLS VSMIRKYARL IQKGNDAIFN 851 SKMIAQQLNN LLLCLSHREP TLQDAAFHAC EIFIRSIPSS LCHHLSLYTL 901 LDMLTALFDS ILDSEAHKFE PRYEFKLKHS KTTILVPSSS SWRATTLSRL 951 HKSAKEWVRI LLNRSNQDTK ILLQSYISDL GEYSRLNSVE FGVSFAMDMA 1001 GLILPADKEL SRLTYYGPEK PNTISGFISL HSWRSKYLFD TAITSSPEDI 1051 KRQIGISTQN IRKNLTLGNK IITKDVTDFL DMATALLILG NGAPASLIYD 1101 IVHIPFEVFT SASLKIATNV WLTIITEKPE VAHLLLVEVC YCWMRSIDDN 1151 IGLYSRDHDL KGEEYQKMEY SPYDKAGINR DAKNASQAMQ PHLHVIKFFA 1201 SHFEGTLFQS DFLLKIFTKC ALYGIKNLYK ASLHPFARMI RHELLLFATL 1251 VLNASYKQGS KYMGRLSQEI TNGALSWFKR PVAWPFGSNE LKIKADLSVT 1301 RDLFLQLNKL SSLMSRHCGK DYKILNYFLA SEIQQIQTWL TPTEKIEGAD 1351 SNELTSDIVE ATFAKDPTLA INLLQRCYSK KAEDVLVGLV AKHALMCVGS 1401 PSALDLFIKG SHLSSKKDLH ATLYWAPVSP LKSINLFLPE WQGNSFILQF 1451 SIYSLESQDV NLAFFYVPQI VQCLRYDKTG YVERLILDTA KISVLFSHQI 1501 IWNMLANCYK DDEGIQEDEI KPTLDRIRER MVSSFSQSHR DFYEREFEFF 1551 DEVTGISGKL KPYIKKSKAE KKHKIDEEMS KIEVKPDVYL PSNPDGVVID 1601 IDRKSGKPLQ SHAKAPFMAT FKIKKDVKDP LTGKNKEVEK WQAAIFKVGD 1651 DCRQDVLALQ LISLFRTIWS SIGLDVYVFP YRVTATAPGC GVIDVLPNSV 1701 SRDMLGREAV NGLYEYFTSK FGNESTIEFQ NARNNFVKSL AGYSVISYLL 1751 QFKDRHNGNI MYDDQGHCLH IDFGFIFDIV PGGIKFEAVP FKLTKEMVKV 1801 MGGSPQTPAY LDFEELCIKA YLAARPHVEA IIECVNPMLG SGLPCFKGHK 1851 TIRNLRARFQ PQKTDHEAAL YMKALIRKSY ESIFTKGYDE FQRLTNGIPY 1901 *

Amino Acid Sequence (or in FASTA format)

       1  MRFTRGLKAS SSLRAKAIGR LTKLSTGAPN DQNSNGTTLD LITHTLPIFY
      51  STNTSKIYTI PLTLSEWEVL TSLCVAIPTT LDLVETMLKE IIAPYFLETP
     101  RQRISDVLSS KFKLEQMRNP IELLTFQLTK FMIQACEQYP VLYENIGGII
     151  STYFERVLKI FTIKQSGLLS LVGFINAFIQ FPNSTELTKF TWKKLAKLVL
     201  RGSFLNEVDK ILNSSATFTN DSIVQYYDAG NELSSAYLLE LISRLQVSLI
     251  SHLLNTSHVG ANLSEFLLNQ QYQFYKFDQE VADENDDTKC IDDFFFNVRS
     301  NKQFFTDMCK ISLQFCSESH ILDLSTDNRA RFSFDTRAHY LQTLCLIPFI
     351  EDTESELFES FTNVVSESID KFFLSDVVTP SLIKAIVASA SLLNFFTEKL
     401  SLTLIRMFPL LVASPHITTE TVNDVAKIFT TGLYPLNEDA IVSTIYSMNN
     451  LLAVSEDGSP VPVLRERQLT ITSGKNIEKD YFPLRNSSAS LDGTGALLGN
     501  TTVGQLSSHD VNSGATMTYH ASLISNCVAA TTTIASYYNT QSITALTISI
     551  LTQKVNSMSK ELDGVILNSL ARLAPNTSLT EFSLLLKFFK SRTVIATKID
     601  DSALLKNIIK AKCVISKELL ARHFSSDLYF MYLHDLLDSI IASGEVERLE
     651  HHRPQTEISR VADQIATYLE PLAALLPVPG DTPLDINKDE VTTNKFRNAW
     701  FNFVIHGYHL GGPIVKRNFS FLLTIAYNSP PLASEFPANN KELSLEMNTI
     751  LRRGSSNENI KQQKQQITEY FNTNIVQYRT TSSSKIMFLA AAVLLETIRC
     801  EAGDCSKTLL YFSDPSILSG SIEKCIAVLS VSMIRKYARL IQKGNDAIFN
     851  SKMIAQQLNN LLLCLSHREP TLQDAAFHAC EIFIRSIPSS LCHHLSLYTL
     901  LDMLTALFDS ILDSEAHKFE PRYEFKLKHS KTTILVPSSS SWRATTLSRL
     951  HKSAKEWVRI LLNRSNQDTK ILLQSYISDL GEYSRLNSVE FGVSFAMDMA
    1001  GLILPADKEL SRLTYYGPEK PNTISGFISL HSWRSKYLFD TAITSSPEDI
    1051  KRQIGISTQN IRKNLTLGNK IITKDVTDFL DMATALLILG NGAPASLIYD
    1101  IVHIPFEVFT SASLKIATNV WLTIITEKPE VAHLLLVEVC YCWMRSIDDN
    1151  IGLYSRDHDL KGEEYQKMEY SPYDKAGINR DAKNASQAMQ PHLHVIKFFA
    1201  SHFEGTLFQS DFLLKIFTKC ALYGIKNLYK ASLHPFARMI RHELLLFATL
    1251  VLNASYKQGS KYMGRLSQEI TNGALSWFKR PVAWPFGSNE LKIKADLSVT
    1301  RDLFLQLNKL SSLMSRHCGK DYKILNYFLA SEIQQIQTWL TPTEKIEGAD
    1351  SNELTSDIVE ATFAKDPTLA INLLQRCYSK KAEDVLVGLV AKHALMCVGS
    1401  PSALDLFIKG SHLSSKKDLH ATLYWAPVSP LKSINLFLPE WQGNSFILQF
    1451  SIYSLESQDV NLAFFYVPQI VQCLRYDKTG YVERLILDTA KISVLFSHQI
    1501  IWNMLANCYK DDEGIQEDEI KPTLDRIRER MVSSFSQSHR DFYEREFEFF
    1551  DEVTGISGKL KPYIKKSKAE KKHKIDEEMS KIEVKPDVYL PSNPDGVVID
    1601  IDRKSGKPLQ SHAKAPFMAT FKIKKDVKDP LTGKNKEVEK WQAAIFKVGD
    1651  DCRQDVLALQ LISLFRTIWS SIGLDVYVFP YRVTATAPGC GVIDVLPNSV
    1701  SRDMLGREAV NGLYEYFTSK FGNESTIEFQ NARNNFVKSL AGYSVISYLL
    1751  QFKDRHNGNI MYDDQGHCLH IDFGFIFDIV PGGIKFEAVP FKLTKEMVKV
    1801  MGGSPQTPAY LDFEELCIKA YLAARPHVEA IIECVNPMLG SGLPCFKGHK
    1851  TIRNLRARFQ PQKTDHEAAL YMKALIRKSY ESIFTKGYDE FQRLTNGIPY
    1901  *

external links for Stt4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Stt4p

1)	Yoshida S, et al. (1994) A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae. J Biol Chem 269(2):1166-72
2)	Pramanik A, et al. (1997) Cloning, characterization and identification of the gene encoding phosphatidylinositol 4-kinase. Cell Mol Biol (Noisy-le-grand) 43(7):1007-18
3)	Audhya A, et al. (2000) Distinct roles for the yeast phosphatidylinositol 4-kinases, Stt4p and Pik1p, in secretion, cell growth, and organelle membrane dynamics. Mol Biol Cell 11(8):2673-89
4)	Audhya A and Emr SD (2002) Stt4 PI 4-kinase localizes to the plasma membrane and functions in the Pkc1-mediated MAP kinase cascade. Dev Cell 2(5):593-605
5)	Yoshida S, et al. (1992) Characterization of a staurosporine- and temperature-sensitive mutant, stt1, of Saccharomyces cerevisiae: STT1 is allelic to PKC1. Mol Gen Genet 231(3):337-44
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41