GSP1/YLR293C Protein Information Help

Standard Name Gsp1p
Systematic Name Ylr293cp
Alias Cnr1p 1 , Cst17p 2
ORF Classification Verified
Description Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication (1, 10, 3, 4, 5, 6, 7, 8, 9)
Name Description Genetic Suppressor of Prp20-1 3
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 219
Molecular Weight (Da) 24,810
Isoelectric Point (pI) 6.52

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gsp1p (InterPro)
Physical Interactions There are 185 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012BC16 | P32835
MIPS: YLR293C
NCBI: 171621 | 256599885 | 256599888 | 288965342 | 297343063 | 311752 | 417090 | 45270616 | 596049 | 6323324 | NP_013396.1 | NM_001182181.1
GenBank/EMBL/DDBJ: DAA09605.1 | AY558363 | L08690 | U17243 | X71945
Amino Acid Sequence (or in FASTA format)
 
       1  MSAPAANGEV PTFKLVLVGD GGTGKTTFVK RHLTGEFEKK YIATIGVEVH
      51  PLSFYTNFGE IKFDVWDTAG QEKFGGLRDG YYINAQCAII MFDVTSRITY
     101  KNVPNWHRDL VRVCENIPIV LCGNKVDVKE RKVKAKTITF HRKKNLQYYD
     151  ISAKSNYNFE KPFLWLARKL AGNPQLEFVA SPALAPPEVQ VDEQLMQQYQ
     201  QEMEQATALP LPDEDDADL*

external links for Gsp1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Mass Spec (Seattle)


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gsp1p
1) Kadowaki T, et al.  (1993) Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. EMBO J 12(7):2929-37
2) Ouspenski II, et al.  (1995) Ran-binding protein-1 is an essential component of the Ran/RCC1 molecular switch system in budding yeast. J Biol Chem 270(5):1975-8
3) Belhumeur P, et al.  (1993) GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization. Mol Cell Biol 13(4):2152-61
4) Schlenstedt G, et al.  (1997) Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus. EMBO J 16(20):6237-49
5) Kunzler M and Hurt EC  (1998) Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett 433(3):185-90
6) Becker J, et al.  (1995) RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J Biol Chem 270(20):11860-5
7) Lee A, et al.  (1994) Site-directed mutagenesis of the yeast PRP20/SRM1 gene reveals distinct activity domains in the protein product. Mol Gen Genet 245(1):32-44
8) Schlenstedt G, et al.  (1995) Mutants in a yeast Ran binding protein are defective in nuclear transport. EMBO J 14(21):5367-78
9) Floer M and Blobel G  (1996) The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein. J Biol Chem 271(10):5313-6
10) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61