GUF1/YLR289W Protein Information

Standard Name	Guf1p
Systematic Name	Ylr289wp
ORF Classification	Verified
Description	Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor (1, 2)
Name Description	Gtpase of Unknown Function 1

Experimental Data
Molecules/cell	1890 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	645
Molecular Weight (Da)	73,188
Isoelectric Point (pI)	9.95

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Guf1p (InterPro)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012BD9E \| P46943 MIPS: YLR289W NCBI: 2506379 \| 596037 \| 6323320 \| 722349 \| NP_013392.1 GenBank/EMBL/DDBJ: DAA09601.1 \| U17243 \| U22360
External Classifications	EC: 3.6.5.- [Hydrolases, Acting on GTP; involved in cellular and subcellular movement]

Amino Acid Sequence (or in FASTA format)
1 MLKFRIRPVR HIRCYKRHAY FLRYNHTTTP AQKLQAQIEQ IPLENYRNFS 51 IVAHVDHGKS TLSDRLLEIT HVIDPNARNK QVLDKLEVER ERGITIKAQT 101 CSMFYKDKRT GKNYLLHLID TPGHVDFRGE VSRSYASCGG AILLVDASQG 151 IQAQTVANFY LAFSLGLKLI PVINKIDLNF TDVKQVKDQI VNNFELPEED 201 IIGVSAKTGL NVEELLLPAI IDRIPPPTGR PDKPFRALLV DSWYDAYLGA 251 VLLVNIVDGS VRKNDKVICA QTKEKYEVKD IGIMYPDRTS TGTLKTGQVG 301 YLVLGMKDSK EAKIGDTIMH LSKVNETEVL PGFEEQKPMV FVGAFPADGI 351 EFKAMDDDMS RLVLNDRSVT LERETSNALG QGWRLGFLGS LHASVFRERL 401 EKEYGSKLII TQPTVPYLVE FTDGKKKLIT NPDEFPDGAT KRVNVAAFHE 451 PFIEAVMTLP QEYLGSVIRL CDSNRGEQID ITYLNTNGQV MLKYYLPLSH 501 LVDDFFGKLK SVSRGFASLD YEDAGYRISD VVKLQLLVNG NAIDALSRVL 551 HKSEVERVGR EWVKKFKEYV KSQLYEVVIQ ARANNKIIAR ETIKARRKDV 601 LQKLHASDVS RRKKLLAKQK EGKKHMKTVG NIQINQEAYQ AFLRR*

Amino Acid Sequence (or in FASTA format)

       1  MLKFRIRPVR HIRCYKRHAY FLRYNHTTTP AQKLQAQIEQ IPLENYRNFS
      51  IVAHVDHGKS TLSDRLLEIT HVIDPNARNK QVLDKLEVER ERGITIKAQT
     101  CSMFYKDKRT GKNYLLHLID TPGHVDFRGE VSRSYASCGG AILLVDASQG
     151  IQAQTVANFY LAFSLGLKLI PVINKIDLNF TDVKQVKDQI VNNFELPEED
     201  IIGVSAKTGL NVEELLLPAI IDRIPPPTGR PDKPFRALLV DSWYDAYLGA
     251  VLLVNIVDGS VRKNDKVICA QTKEKYEVKD IGIMYPDRTS TGTLKTGQVG
     301  YLVLGMKDSK EAKIGDTIMH LSKVNETEVL PGFEEQKPMV FVGAFPADGI
     351  EFKAMDDDMS RLVLNDRSVT LERETSNALG QGWRLGFLGS LHASVFRERL
     401  EKEYGSKLII TQPTVPYLVE FTDGKKKLIT NPDEFPDGAT KRVNVAAFHE
     451  PFIEAVMTLP QEYLGSVIRL CDSNRGEQID ITYLNTNGQV MLKYYLPLSH
     501  LVDDFFGKLK SVSRGFASLD YEDAGYRISD VVKLQLLVNG NAIDALSRVL
     551  HKSEVERVGR EWVKKFKEYV KSQLYEVVIQ ARANNKIIAR ETIKARRKDV
     601  LQKLHASDVS RRKKLLAKQK EGKKHMKTVG NIQINQEAYQ AFLRR*

external links for Guf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Guf1p

1)	Kiser GL and Weinert TA (1995) GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast. Yeast 11(13):1311-6
2)	Bauerschmitt H, et al. (2008) The Membrane-bound GTPase Guf1 Promotes Mitochondrial Protein Synthesis under Suboptimal Conditions. J Biol Chem 283(25):17139-46
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41