MEC3/YLR288C Protein Information Help

Standard Name Mec3p
Systematic Name Ylr288cp
Alias Pip3p , Pso9p 1
ORF Classification Verified
Description DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 (2, 3, 4, 5, 6)
Name Description Mitosis Entry Checkpoint 7
Experimental Data
Molecules/cell 414 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 474
Molecular Weight (Da) 53,145
Isoelectric Point (pI) 5.1

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mec3p (InterPro)
Physical Interactions There are 43 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001683A7 | Q02574
MIPS: YLR288C
NCBI: 108935851 | 2209256 | 2498537 | 596045 | 6323319 | NP_013391.1
GenBank/EMBL/DDBJ: DAA09600.1 | U17243 | U30906
Amino Acid Sequence (or in FASTA format)
 
       1  MKLKLIVNGC EAPDDYKLLR TTINTVASLR KTAILRFNSE RLTIISTPKS
      51  SLNSSNNGTI LRGDTGQLWC TIPHDVFRLY TVISARELNT ITMECNCDSL
     101  LSVFKRYDRV MNQGSSSNMT IKLQSMPEWN TNNGTLSGGT AGGVDTTSKP
     151  NPICALGITF EEIVHTSGPN DAIVMNGGVD EHNGLPTTVG TGNLLASNKV
     201  IMHSFKVPVK LLFRAQDTRI QEPMINYIQL MMYKLPPISG EFGSAFHGFI
     251  RRVERYSNVN HIHLMGVKKK EHGNEGDDVE LKIIVNELDW HLEICWNGPL
     301  DSVIQRQEGL TDNPSQNQHI DTDGRQEEGS LPIIEADKPM SSLYTNTRDR
     351  EMEENIRYDE DLLRIEDSSI ADTRGNIYTA DTSGDTEFND ISVMVEKAEQ
     401  ESSSTHEVII RCKDWKVCSK LYAAFEEVVL AISHDESCVF HCSLDRGSLE
     451  DSEDVEKPRE RGQIIYYIAR SKGL*

external links for Mec3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome

YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mec3p
1) Cardone JM, et al.  (2006) Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. DNA Repair (Amst) 5(2):163-71
2) Kondo T, et al.  (1999) Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway. Mol Cell Biol 19(2):1136-43
3) de la Torre-Ruiz M and Lowndes NF  (2000) The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining. FEBS Lett 467(2-3):311-5
4) Hong EJ and Roeder GS  (2002) A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev 16(3):363-76
5) Majka J and Burgers PM  (2003) Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint. Proc Natl Acad Sci U S A 100(5):2249-54
6) Rouse J and Jackson SP  (2002) Interfaces between the detection, signaling, and repair of DNA damage. Science 297(5581):547-51
7) Weinert TA, et al.  (1994) Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair. Genes Dev 8(6):652-65
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41