NNT1/YLR285W Protein Information

Standard Name	Nnt1p 1
Systematic Name	Ylr285wp
ORF Classification	Verified
Description	S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination (1, 2)
Name Description	Nicotinamide N-methylTransferase

Experimental Data
Molecules/cell	7280 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	261
Molecular Weight (Da)	29,632
Isoelectric Point (pI)	4.88

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nnt1p (InterPro)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000697F5 \| Q05874 MIPS: YLR285W NCBI: 51013527 \| 596038 \| 6323315 \| 74644920 \| NP_013387.1 GenBank/EMBL/DDBJ: DAA09595.1 \| AY693038 \| U17243
External Classifications	EC: 2.1.1.1 [Nicotinamide N-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL 51 RLVGTSPLWG HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC 101 ALNGAQMVVS TDYPDPDLMQ NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP 151 LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH HKLLQTTKDL LAEKGQALVV 201 FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD EDEETIEVRS 251 RVYAYYLTHE K*

Amino Acid Sequence (or in FASTA format)

       1  MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL
      51  RLVGTSPLWG HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC
     101  ALNGAQMVVS TDYPDPDLMQ NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP
     151  LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH HKLLQTTKDL LAEKGQALVV
     201  FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD EDEETIEVRS
     251  RVYAYYLTHE K*

external links for Nnt1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Nnt1p

1)	Anderson RM, et al. (2003) Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature 423(6936):181-5
2)	Wlodarski T, et al. (2011) Comprehensive Structural and Substrate Specificity Classification of the Saccharomyces cerevisiae Methyltransferome. PLoS One 6(8):e23168
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41