MCM5/YLR274W Protein Information Help

Standard Name Mcm5p 1
Systematic Name Ylr274wp
Alias Bob1p 2 , Cdc46p 3
ORF Classification Verified
Description Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase (4)
Name Description MiniChromosome Maintenance 1
Experimental Data
Molecules/cell 10300 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 775
Molecular Weight (Da) 86,410
Isoelectric Point (pI) 5.49

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mcm5p (InterPro)
Physical Interactions There are 108 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000531AD | P29496
MIPS: YLR274W
NCBI: 231717 | 485273 | 577187 | 6323304 | NP_013376.1 | NM_001182161.1
GenBank/EMBL/DDBJ: DAA09587.1 | U09242 | U17245
External Classifications EC: 3.6.4.12 [DNA helicase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL
      51  RNNILVKNYS LTVNMEHLIG YNEDIYKKLS DEPSDIIPLF ETAITQVAKR
     101  ISILSRAQSA NNNDKDPENT SMDTDSLLLN SLPTFQLILN SNANQIPLRD
     151  LDSEHVSKIV RLSGIIISTS VLSSRATYLS IMCRNCRHTT SITINNFNSI
     201  TGNTVSLPRS CLSTIESESS MANESNIGDE STKKNCGPDP YIIIHESSKF
     251  IDQQFLKLQE IPELVPVGEM PRNLTMTCDR YLTNKVIPGT RVTIVGIYSI
     301  YNSKNGAGSG RSGGGNGGSG VAIRTPYIKI LGIQSDVETS SIWNSVTMFT
     351  EEEEEEFLQL SRNPKLYEIL TNSIAPSIFG NEDIKKAIVC LLMGGSKKIL
     401  PDGMRLRGDI NVLLLGDPGT AKSQLLKFVE KVSPIAVYTS GKGSSAAGLT
     451  ASVQRDPMTR EFYLEGGAMV LADGGVVCID EFDKMRDEDR VAIHEAMEQQ
     501  TISIAKAGIT TVLNSRTSVL AAANPIYGRY DDLKSPGDNI DFQTTILSRF
     551  DMIFIVKDDH NEERDISIAN HVINIHTGNA NAMQNQQEEN GSEISIEKMK
     601  RYITYCRLKC APRLSPQAAE KLSSNFVTIR KQLLINELES TERSSIPITI
     651  RQLEAIIRIT ESLAKLELSP IAQERHVDEA IRLFQASTMD AASQDPIGGL
     701  NQASGTSLSE IRRFEQELKR RLPIGWSTSY QTLRREFVDT HRFSQLALDK
     751  ALYALEKHET IQLRHQGQNI YRSGV*

external links for Mcm5p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Mcm5p
1) Chen Y, et al.  (1992) CDC46/MCM5, a yeast protein whose subcellular localization is cell cycle-regulated, is involved in DNA replication at autonomously replicating sequences. Proc Natl Acad Sci U S A 89(21):10459-63
2) Hardy CF, et al.  (1997) mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p. Proc Natl Acad Sci U S A 94(7):3151-5
3) Moir D, et al.  (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
4) Tye BK  (1999) MCM proteins in DNA replication. Annu Rev Biochem 68:649-86
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41