HAP1/YLR256W Protein Information Help

Standard Name Hap1p
Systematic Name Ylr256wp
Alias Cyp1p 1
ORF Classification Verified
Description Zinc finger transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus (2, 3, 4, 5, 6)
Name Description Heme Activator Protein 3, 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,502
Molecular Weight (Da) 166,106
Isoelectric Point (pI) 7.35

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hap1p (InterPro)
Physical Interactions There are 19 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000069925 | P0CE41
MIPS: YLR256W
NCBI: 290463258 | 6323285 | 662331 | NP_013357.1 | NM_001182143.1
GenBank/EMBL/DDBJ: DAA09568.1 | U20865
Amino Acid Sequence (or in FASTA format)
 
       1  MSNTPYNSSV PSIASMTQSS VSRSPNMHTA TTPGANTSSN SPPLHMSSDS
      51  SKIKRKRNRI PLSCTICRKR KVKCDKLRPH CQQCTKTGVA HLCHYMEQTW
     101  AEEAEKELLK DNELKKLRER VKSLEKTLSK VHSSPSSNSL KSYNTPESSN
     151  LFMGSDEHTT LVNANTGSAS SASHMHQQQQ QQQQQEQQQD FSRSANANAN
     201  SSSLSISNKY DNDELDLTKD FDLLHIKSNG TIHLGATHWL SIMKGDPYLK
     251  LLWGHIFAMR EKLNEWYYQK NSYSKLKSSK CPINHAQAPP SAAAAATRKC
     301  PVDHSAFSSG MVAPKEETPL PRKCPVDHTM FSSGMIPPRE DTSSQKRCPV
     351  DHTMYSAGMM PPKDETPSPF STKAMIDHNK HTMNPPQSKC PVDHRNYMKD
     401  YPSDMANSSS NPASRCPIDH SSMKNTAALP ASTHNTIPHH QPQSGSHARS
     451  HPAQSRKHDS YMTESEVLAT LCEMLPPKRV IALFIEKFFK HLYPAIPILD
     501  EQNFKNHVNQ MLSLSSMNPT VNNFGMSMPS SSTLENQPIT QINLPKLSDS
     551  CNLGILIIIL RLTWLSIPSN SCEVDLGEES GSFLVPNESS NMSASALTSM
     601  AKEESLLLKH ETPVEALELC QKYLIKFDEL SSISNNNVNL TTVQFAIFYN
     651  FYMKSASNDL TTLTNTNNTG MANPGHDSES HQILLSNITQ MAFSCGLHRD
     701  PDNFPQLNAT IPATSQDVSN NGSKKANPST NPTLNNNMSA ATTNSSSRSG
     751  SADSRSGSNP VNKKENQVSI ERFKHTWRKI WYYIVSMDVN QSLSLGSPRL
     801  LRNLRDFSDT KLPSASRIDY VRDIKELIIV KNFTLFFQID LCIIAVLNHI
     851  LNVSLARSVR KFELDSLINL LKNLTYGTEN VNDVVSSLIN KGLLPTSEGG
     901  SVDSNNDEIY GLPKLPDILN HGQHNQNLYA DGRNTSSSDI DKKLDLPHES
     951  TTRALFFSKH MTIRMLLYLL NYILFTHYEP MGSEDPGTNI LAKEYAQEAL
    1001  NFAMDGYRNC MIFFNNIRNT NSLFDYMNVI LSYPCLDIGH RSLQFIVCLI
    1051  LRAKCGPLTG MRESSIITNG TSSGFNSSVE DEDVKVKQES SDELKKDDFM
    1101  KDVNLDSGDS LAEILMSRML LFQKLTKQLS KKYNYAIRMN KSTGFFVSLL
    1151  DTPSKKSDSK SGGSSFMLGN WKHPKVSNMS GFLAGDKDQL QKCPVYQDAL
    1201  GFVSPTGANE GSAPMQGMSL QGSTARMGGT QLPPIRSYKP ITYTSSNLRR
    1251  MNETGEAEAK RRRFNDGYID NNSNNDIPRG ISPKPSNGLS SVQPLLSSFS
    1301  MNQLNGGTIP TVPSLTNITS QMGALPSLDR ITTNQINLPD PSRDEAFDNS
    1351  IKQMTPMTSA FMNANTTIPS STLNGNMNMN GAGTANTDTS ANGSALSTLT
    1401  SPQGSDLASN SATQYKPDLE DFLMQNSNFN GLMINPSSLV EVVGGYNDPN
    1451  NLGRNDAVDF LPVDNVEIDG VGIKINYHLL TSIYVTSILS YTVLEDDAND
    1501  EK*

external links for Hap1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
YGOB CYC2008 (complexes) Pfam domains
YOGY Complexome YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Hap1p
1) Verdiere J, et al.  (1985) Regulation of the expression of iso 2-cytochrome c gene in S. cerevisiae: cloning of the positive regulatory gene CYP1 and identification of the region of its target sequence on the structural gene CYP3. Mol Gen Genet 199(3):524-33
2) Keng T  (1992) HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Mol Cell Biol 12(6):2616-23
3) Pfeifer K, et al.  (1989) Functional dissection and sequence of yeast HAP1 activator. Cell 56(2):291-301
4) Pfeifer K, et al.  (1987) Yeast HAP1 activator binds to two upstream activation sites of different sequence. Cell 49(1):19-27
5) Gaisne M, et al.  (1999) A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1). Curr Genet 36(4):195-200
6) Kwast KE, et al.  (1998) Oxygen sensing and the transcriptional regulation of oxygen-responsive genes in yeast. J Exp Biol 201(Pt 8):1177-95
7) Guarente L, et al.  (1984) Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell 36(2):503-11