RCK2/YLR248W Protein Information Help

Standard Name Rck2p 1
Systematic Name Ylr248wp
Alias Clk1p 2 , Cmk3p 3
ORF Classification Verified
Description Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication (2, 4, 5, 6, 7)
Name Description Radiation sensitivity Complementing Kinase 1
Experimental Data
Molecules/cell 1790 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 610
Molecular Weight (Da) 68,061
Isoelectric Point (pI) 5.18

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rck2p (InterPro)
Physical Interactions There are 63 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001683A2 | P38623
MIPS: YLR248W
NCBI: 2507200 | 308153567 | 467608 | 6323277 | 662336 | 733594 | NP_013349.1
GenBank/EMBL/DDBJ: DAA09562.1 | U20865 | U23464 | X71065
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MLKIKALFSK KKPDQADLSQ ESKKPFKGKT RSSGTNNKDV SQITSSPKKS
      51  FQDKNIVQYP SVVADDHHMK SLTDELVTTI DSDSSPSDNI TTENVETVTS
     101  VPAIDVHESS EGQLSSDPLI SDESLSEQSE IISDIQDDST DDDNMEDEIP
     151  EKSFLEQKEL IGYKLINKIG EGAFSKVFRA IPAKNSSNEF LTKNYKAVAI
     201  KVIKKADLSS INGDHRKKDK GKDSTKTSSR DQVLKEVALH KTVSAGCSQI
     251  VAFIDFQETD SYYYIIQELL TGGEIFGEIV RLTYFSEDLS RHVIKQLALA
     301  VKHMHSLGVV HRDIKPENLL FEPIEFTRSI KPKLRKSDDP QTKADEGIFT
     351  PGVGGGGIGI VKLADFGLSK QIFSKNTKTP CGTVGYTAPE VVKDEHYSMK
     401  VDMWGIGCVL YTMLCGFPPF YDEKIDTLTE KISRGEYTFL KPWWDEISAG
     451  AKNAVAKLLE LEPSKRYDID QFLDDPWLNT FDCLPKEGES SQKKAGTSER
     501  RHPHKKQFQL FQRDSSLLFS PAAVAMRDAF DIGNAVKRTE EDRMGTRGGL
     551  GSLAEDEELE DSYSGAQGDE QLEQNMFQLT LDTSTILQRR KKVQENDVGP
     601  TIPISATIRE *

external links for Rck2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Rck2p
1) Dahlkvist A and Sunnerhagen P  (1994) Two novel deduced serine/threonine protein kinases from Saccharomyces cerevisiae. Gene 139(1):27-33
2) Melcher ML and Thorner J  (1996) Identification and characterization of the CLK1 gene product, a novel CaM kinase-like protein kinase from the yeast Saccharomyces cerevisiae. J Biol Chem 271(47):29958-68
3) Hunter T and Plowman GD  (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
4) Dahlkvist A, et al.  (1995) The RCK1 and RCK2 protein kinase genes from Saccharomyces cerevisiae suppress cell cycle checkpoint mutations in Schizosaccharomyces pombe. Mol Gen Genet 246(3):316-26
5) Bilsland E, et al.  (2004) Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance. Mol Microbiol 53(6):1743-56
6) Teige M, et al.  (2001) Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast. Proc Natl Acad Sci U S A 98(10):5625-30
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41