VPS34/YLR240W Protein Information Help

Standard Name Vps34p 1, 2
Systematic Name Ylr240wp
Alias End12p 3 , Pep15p 4 , Vpl7p 5 , Vpt29p 1 , Stt8p 6, 7 , Vps7p 8
ORF Classification Verified
Description Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery (10, 11, 12, 9)
Name Description Vacuolar Protein Sorting 1, 2
Experimental Data
Molecules/cell 1080 13
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 875
Molecular Weight (Da) 100,920
Isoelectric Point (pI) 7.79

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Vps34p (InterPro)
Physical Interactions There are 19 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000002F88C | P22543
MIPS: YLR240W
NCBI: 12656857 | 139216 | 4784 | 6323269 | 662340 | NP_013341.1
GenBank/EMBL/DDBJ: DAA09554.1 | U19027 | U20865 | X53531
External Classifications EC: 2.7.1.137 [Phosphatidylinositol 3-kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSLNNITFCV SQDLDVPLKV KIKSLEGHKP LLKPSQKILN PELMLIGSNV
      51  FPSSDLIVSL QVFDKERNRN LTLPIYTPYI PFRNSRTWDY WLTLPIRIKQ
     101  LTFSSHLRII LWEYNGSKQI PFFNLETSIF NLKDCTLKRG FESLKFRYDV
     151  IDHCEVVTDN KDQENLNKYF QGEFTRLPWL DEITISKLRK QRENRTWPQG
     201  TFVLNLEFPM LELPVVFIER EIMNTQMNIP TLKNNPGLST DLREPNRNDP
     251  QIKISLGDKY HSTLKFYDPD QPNNDPIEEK YRRLERASKN ANLDKQVKPD
     301  IKKRDYLNKI INYPPGTKLT AHEKGSIWKY RYYLMNNKKA LTKLLQSTNL
     351  REESERVEVL ELMDSWAEID IDDALELLGS TFKNLSVRSY AVNRLKKASD
     401  KELELYLLQL VEAVCFENLS TFSDKSNSEF TIVDAVSSQK LSGDSMLLST
     451  SHANQKLLKS ISSESETSGT ESLPIVISPL AEFLIRRALV NPRLGSFFYW
     501  YLKSESEDKP YLDQILSSFW SRLDKKSRNI LNDQVRLINV LRECCETIKR
     551  LKDTTAKKME LLVHLLETKV RPLVKVRPIA LPLDPDVLIC DVCPETSKVF
     601  KSSLSPLKIT FKTTLNQPYH LMFKVGDDLR QDQLVVQIIS LMNELLKNEN
     651  VDLKLTPYKI LATGPQEGAI EFIPNDTLAS ILSKYHGILG YLKLHYPDEN
     701  ATLGVQGWVL DNFVKSCAGY CVITYILGVG DRHLDNLLVT PDGHFFHADF
     751  GYILGQDPKP FPPLMKLPPQ IIEAFGGAES SNYDKFRSYC FVAYSILRRN
     801  AGLILNLFEL MKTSNIPDIR IDPNGAILRV RERFNLNMSE EDATVHFQNL
     851  INDSVNALLP IVIDHLHNLA QYWRT*

external links for Vps34p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Vps34p
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Munn AL and Riezman H  (1994) Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol 127(2):373-86
4) Stepp JD, et al.  (1997) The yeast adaptor protein complex, AP-3, is essential for the efficient delivery of alkaline phosphatase by the alternate pathway to the vacuole. J Cell Biol 139(7):1761-74
5) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
6) Yoshida S, et al.  (1992) Characterization of a staurosporine- and temperature-sensitive mutant, stt1, of Saccharomyces cerevisiae: STT1 is allelic to PKC1. Mol Gen Genet 231(3):337-44
7) Yoshida S and Anraku Y  (2000) Characterization of staurosporine-sensitive mutants of Saccharomyces cerevisiae: vacuolar functions affect staurosporine sensitivity. Mol Gen Genet 263(5):877-88
8) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
9) Herman PK and Emr SD  (1990) Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Mol Cell Biol 10(12):6742-54
10) Stack JH, et al.  (1993) A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole. EMBO J 12(5):2195-204
11) Burda P, et al.  (2002) Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase. J Cell Sci 115(Pt 20):3889-900
12) Slessareva JE, et al.  (2006) Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome. Cell 126(1):191-203
13) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41