THI7/YLR237W Protein Information

Standard Name	Thi7p 1
Systematic Name	Ylr237wp
Alias	Thi10p 2
ORF Classification	Verified
Description	Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia (1, 2, 3)
Name Description	THIamine metabolism

Experimental Data
Molecules/cell	3120 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	598
Molecular Weight (Da)	66,903
Isoelectric Point (pI)	7.04

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Thi7p (InterPro)
Transmembrane Domains	There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136D8F \| Q05998 MIPS: YLR237W NCBI: 1871134 \| 2492817 \| 609365 \| 6323266 \| NP_013338.1 GenBank/EMBL/DDBJ: DAA09551.1 \| D55634 \| U19027
External Classifications	TC: 2.A.39.4

Amino Acid Sequence (or in FASTA format)
1 MSFGSKVSRA LRFLEIPVKD RASVSFLKNP DLQPIKSANQ TWGFWSNFAY 51 WGVMSFSVGT WMSASSALGV GLSYPETIGT FIVGDVLTII FTLANSCPGY 101 DWKVGFTLAQ RFVFGIYGSA FGIIIRILMS IVNYGSNAWV GGLCINMILD 151 SWSHHYLHLP NTLSSKVAMT TKELIGFIIF HVLTAFCYLM KPYHMNYILI 201 WSCVATFFSM LGMVIYLAKQ AHGVGELFTS TKSTATGSTK AWAWVYMISY 251 WFGSVSPGST NQSDYSRFGS SNWAIWAGTI CALLIPTTLI PVFGVIGAST 301 CDKLYGEQYW MPMDIFNHWL TTNYSAGARA GAFFCGLSFV LSQMSYTISN 351 CGFASGMDLA GLLPKYVDIK RGALFAACVS WACLPWNFYN SSSTFLTVMS 401 SFGVVMTPII SVMICDNFLI RKRQYSITNA FILKGEYYFT KGVNWRAIVA 451 WVCGMTPGLP GIAWEVNNDY FHNTGIVNFF YGDSFFSFLI SFFVYWGLCL 501 LFPFKITVKH DDKDYYGAFT DEEARKKGMV PYSEISEEEI RAYTLGEGYT 551 TGHEYRPEGS DDEIPELVKT SSENTNEFEI VHHKNNEKQS STASEKAA*

Amino Acid Sequence (or in FASTA format)

       1  MSFGSKVSRA LRFLEIPVKD RASVSFLKNP DLQPIKSANQ TWGFWSNFAY
      51  WGVMSFSVGT WMSASSALGV GLSYPETIGT FIVGDVLTII FTLANSCPGY
     101  DWKVGFTLAQ RFVFGIYGSA FGIIIRILMS IVNYGSNAWV GGLCINMILD
     151  SWSHHYLHLP NTLSSKVAMT TKELIGFIIF HVLTAFCYLM KPYHMNYILI
     201  WSCVATFFSM LGMVIYLAKQ AHGVGELFTS TKSTATGSTK AWAWVYMISY
     251  WFGSVSPGST NQSDYSRFGS SNWAIWAGTI CALLIPTTLI PVFGVIGAST
     301  CDKLYGEQYW MPMDIFNHWL TTNYSAGARA GAFFCGLSFV LSQMSYTISN
     351  CGFASGMDLA GLLPKYVDIK RGALFAACVS WACLPWNFYN SSSTFLTVMS
     401  SFGVVMTPII SVMICDNFLI RKRQYSITNA FILKGEYYFT KGVNWRAIVA
     451  WVCGMTPGLP GIAWEVNNDY FHNTGIVNFF YGDSFFSFLI SFFVYWGLCL
     501  LFPFKITVKH DDKDYYGAFT DEEARKKGMV PYSEISEEEI RAYTLGEGYT
     551  TGHEYRPEGS DDEIPELVKT SSENTNEFEI VHHKNNEKQS STASEKAA*

external links for Thi7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	TCDB
	DIP	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Thi7p

1)	Singleton CK (1997) Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae. Gene 199(1-2):111-21
2)	Enjo F, et al. (1997) Isolation and characterization of a thiamin transport gene, THI10, from Saccharomyces cerevisiae. J Biol Chem 272(31):19165-70
3)	Stagg AR, et al. (1999) Defective high-affinity thiamine transporter leads to cell death in thiamine-responsive megaloblastic anemia syndrome fibroblasts. J Clin Invest 103(5):723-9
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41