ECM22/YLR228C Protein Information Help

Standard Name Ecm22p 1
Systematic Name Ylr228cp
ORF Classification Verified
Description Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description ExtraCellular Mutant
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 814
Molecular Weight (Da) 89,285
Isoelectric Point (pI) 6.55

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ecm22p (InterPro)
Physical Interactions There are 9 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006C512 | Q05958
MIPS: YLR228C
NCBI: 60389820 | 609375 | 6323257 | NP_013329.1
GenBank/EMBL/DDBJ: DAA09546.1 | U19027
Amino Acid Sequence (or in FASTA format)
 
       1  MTSDDGNAGQ EREKDAELIE VGGKKVSKTS TGKRKFHNKS KTGCDNCKRR
      51  RVKCDEGKPF CKKCTNMKLD CVYSPIQPRR RKDSSSSKFA SAVHDRVGKK
     101  NLSDNAIMLQ QQQQQLHHQQ EQQFRQQQQV QLQQQLLPHV GTDEQSNSPN
     151  SVPPSVSNNM ENLLLPHLLA SLVNNTSNST NSSANGAEAH NNITQTAPSS
     201  MINNNHPNMA LPGNSPLSIP ITPSFQSTAM NLSSSLNGLL SPGRLNSVTN
     251  GLQQPQLQQQ NQQIPQQQGT QSPFSNIPFD QLAQLNKMGL NFNMKSFNTL
     301  FPYGAANGMA SEFQELFGLG KFATSNNRAI KVSTAEEALA NMQQEQEDKN
     351  KQFTKNPLDN TKTDAVNSGN NPLNGNENKV TASDILSHNK NLIIDNTGLT
     401  ISPPHTLSKP SIDQNIASPS TGVSNVTSTK SLLSIPDNRT ALGNSPTLKT
     451  SPMGDLLSNS EALSPRSSNS HTQQQSSPHS NASSASRLVP ELVGLSRKSN
     501  LNLIDLKLFH HYCTDVWHTI TEAGISGPEV WSTYIPDLAF HFPFLMHTIL
     551  AFSATHLSRT EAGLDNYVSS HRLEALRLLR EAVLEISDDN TDALVASALI
     601  LILDSLANAS SSSPTAWIFH VKGAVTILTA VWPLSETSKF YNLISVDLSD
     651  LGEAVINQSN HNNDNDNSNN GDGNNNNTIS ELVCFDESIA DLYPVEIDSP
     701  YLITLAYLDK LHREKNQLDF MLRVFSFPAL LDRTFLALLM TGDLGAMRIM
     751  RSYYTLLRGY TTEIKDKVWF LDSVSQVLPQ DVDEYSGGGG MHMMLDFLGG
     801  GLPSMTTTNF SAFM*

external links for Ecm22p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Ecm22p
1) Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2) Shianna KV, et al.  (2001) Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake. J Bacteriol 183(3):830-4
3) Vik A and Rine J  (2001) Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol 21(19):6395-405
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Marie C, et al.  (2008) Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae. Fungal Genet Biol 45(10):1430-8