FRE1/YLR214W Protein Information Help

Standard Name Fre1p
Systematic Name Ylr214wp
ORF Classification Verified
Description Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels (1, 2)
Name Description Ferric REductase 3
Experimental Data
Molecules/cell 892 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 686
Molecular Weight (Da) 78,853
Isoelectric Point (pI) 9.86

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Fre1p (InterPro)
Transmembrane Domains There are 7 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 11 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012ABF2 | P32791
MIPS: YLR214W
NCBI: 171521 | 417005 | 544499 | 6323243 | NP_013315.1
GenBank/EMBL/DDBJ: DAA09531.1 | M86908 | U14913
External Classifications EC: 1.16.1.-
EC: 1.16.1.7 [Ferric-chelate reductase (NADH)] TC: 1.A.20.5
Amino Acid Sequence (or in FASTA format)
 
       1  MVRTRVLFCL FISFFATVQS SATLISTSCI SQAALYQFGC SSKSKSCYCK
      51  NINWLGSVTA CAYENSKSNK TLDSALMKLA SQCSSIKVYT LEDMKNIYLN
     101  ASNYLRAPEK SDKKTVVSQP LMANETAYHY YYEENYGIHL NLMRSQWCAW
     151  GLVFFWVAVL TAATILNILK RVFGKNIMAN SVKKSLIYPS VYKDYNERTF
     201  YLWKRLPFNF TTRGKGLVVL IFVILTILSL SFGHNIKLPH PYDRPRWRRS
     251  MAFVSRRADL MAIALFPVVY LFGIRNNPFI PITGLSFSTF NFYHKWSAYV
     301  CFMLAVVHSI VMTASGVKRG VFQSLVRKFY FRWGIVATIL MSIIIFQSEK
     351  VFRNRGYEIF LLIHKAMNIM FIIAMYYHCH TLGWMGWIWS MAGILCFDRF
     401  CRIVRIIMNG GLKTATLSTT DDSNVIKISV KKPKFFKYQV GAFAYMYFLS
     451  PKSAWFYSFQ SHPFTVLSER HRDPNNPDQL TMYVKANKGI TRVLLSKVLS
     501  APNHTVDCKI FLEGPYGVTV PHIAKLKRNL VGVAAGLGVA AIYPHFVECL
     551  RLPSTDQLQH KFYWIVNDLS HLKWFENELQ WLKEKSCEVS VIYTGSSVED
     601  TNSDESTKGF DDKEESEITV ECLNKRPDLK ELVRSEIKLS ELENNNITFY
     651  SCGPATFNDD FRNAVVQGID SSLKIDVELE EESFTW*

external links for Fre1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains

Complexome TCDB

DIP YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Fre1p
1) Georgatsou E and Alexandraki D  (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
2) Yun CW, et al.  (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23
3) Dancis A, et al.  (1990) Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2294-301
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41