NMT1/YLR195C Protein Information

Standard Name	Nmt1p
Systematic Name	Ylr195cp
Alias	Cdc72p 1
ORF Classification	Verified
Description	N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction (2, 3, 4, 5)
Name Description	N-Myristoyl Transferase 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	455
Molecular Weight (Da)	52,837
Isoelectric Point (pI)	7.42

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nmt1p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000130281 \| P14743 MIPS: YLR195C NCBI: 128399 \| 14278241 \| 14278242 \| 14278243 \| 149242913 \| 149242914 \| 149242915 \| 149242916 \| 149242917 \| 149242918 \| 149242921 \| 149242922 \| 149242923 \| 149242924 \| 149242925 \| 149242926 \| 149242929 \| 149242930 \| 149242931 \| 149242932 \| 149242933 \| 149242934 \| 172045 \| 4139448 \| 544511 \| 6323224 \| NP_013296.1 GenBank/EMBL/DDBJ: DAA09514.1 \| M23726 \| U14913
External Classifications	EC: 2.3.1.97 [Glycylpeptide N-tetradecanoyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MSEEDKAKKL ENLLKLLQLN NDDTSKFTQE QKKAMKDHKF WRTQPVKDFD 51 EKVVEEGPID KPKTPEDISD KPLPLLSSFE WCSIDVDNKK QLEDVFVLLN 101 ENYVEDRDAG FRFNYTKEFF NWALKSPGWK KDWHIGVRVK ETQKLVAFIS 151 AIPVTLGVRG KQVPSVEINF LCVHKQLRSK RLTPVLIKEI TRRVNKCDIW 201 HALYTAGIVL PAPVSTCRYT HRPLNWKKLY EVDFTGLPDG HTEEDMIAEN 251 ALPAKTKTAG LRKLKKEDID QVFELFKRYQ SRFELIQIFT KEEFEHNFIG 301 EESLPLDKQV IFSYVVEQPD GKITDFFSFY SLPFTILNNT KYKDLGIGYL 351 YYYATDADFQ FKDRFDPKAT KALKTRLCEL IYDACILAKN ANMDVFNALT 401 SQDNTLFLDD LKFGPGDGFL NFYLFNYRAK PITGGLNPDN SNDIKRRSNV 451 GVVML*

Amino Acid Sequence (or in FASTA format)

       1  MSEEDKAKKL ENLLKLLQLN NDDTSKFTQE QKKAMKDHKF WRTQPVKDFD
      51  EKVVEEGPID KPKTPEDISD KPLPLLSSFE WCSIDVDNKK QLEDVFVLLN
     101  ENYVEDRDAG FRFNYTKEFF NWALKSPGWK KDWHIGVRVK ETQKLVAFIS
     151  AIPVTLGVRG KQVPSVEINF LCVHKQLRSK RLTPVLIKEI TRRVNKCDIW
     201  HALYTAGIVL PAPVSTCRYT HRPLNWKKLY EVDFTGLPDG HTEEDMIAEN
     251  ALPAKTKTAG LRKLKKEDID QVFELFKRYQ SRFELIQIFT KEEFEHNFIG
     301  EESLPLDKQV IFSYVVEQPD GKITDFFSFY SLPFTILNNT KYKDLGIGYL
     351  YYYATDADFQ FKDRFDPKAT KALKTRLCEL IYDACILAKN ANMDVFNALT
     401  SQDNTLFLDD LKFGPGDGFL NFYLFNYRAK PITGGLNPDN SNDIKRRSNV
     451  GVVML*

external links for Nmt1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Nmt1p

1)	Reed SI, et al. (1988) Isolation and characterization of two genes encoding yeast mating pheromone signaling elements: CDC72 and CDC73. Cold Spring Harb Symp Quant Biol 53 Pt 2:621-7
2)	Knoll LJ, et al. (1992) Analysis of the compartmentalization of myristoyl-CoA:protein N-myristoyltransferase in Saccharomyces cerevisiae. J Biol Chem 267(8):5366-73
3)	Stone DE, et al. (1991) N-myristoylation is required for function of the pheromone-responsive G alpha protein of yeast: conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase. Genes Dev 5(11):1969-81
4)	Duronio RJ, et al. (1989) Disruption of the yeast N-myristoyl transferase gene causes recessive lethality. Science 243(4892):796-800
5)	Towler DA, et al. (1987) Amino-terminal processing of proteins by N-myristoylation. Substrate specificity of N-myristoyl transferase. J Biol Chem 262(3):1030-6