TOS4/YLR183C Protein Information

Standard Name	Tos4p 1
Systematic Name	Ylr183cp
ORF Classification	Verified
Description	Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	Target Of Sbf 1

Experimental Data
Molecules/cell	284 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	489
Molecular Weight (Da)	55,467
Isoelectric Point (pI)	10.12

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tos4p (InterPro)
Physical Interactions	There are 11 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005321B \| Q06266 MIPS: YLR183C NCBI: 577214 \| 6323212 \| 74644956 \| NP_013284.1 GenBank/EMBL/DDBJ: DAA09503.1 \| U17246

Amino Acid Sequence (or in FASTA format)
1 MSSQFPSSPY RTVDPYSPPN YKQQPNCPSS NYEKAGKTAS ESIGNFGKGD 51 YPTPFPSSSI GRVSSPVRSN KVDAIPSSPA FPGQLAETSP KFSSKLSSPS 101 RHTRVINAEL DPSKISTITV GRNSSQCDVA LCKNKFISRV HASITYLPQT 151 NEVKIHCFSM NGLIVTYRKQ FDCYQLKDTM NNNNRAYRLV PRFSNEKCVK 201 EIQDEGGFIN FTLEEGDTVY MTYYKGIMLD FRQVLLRISL KEKNSSSEPL 251 RFEKKAEFES ESETKHMGSI RKHPLIFTDT SMDRPKKILK DSNKISIGSD 301 SGVAERMLNH FLNSKSSPLS SVSSVDHEEQ TLRQDSLSSD KNPMTMKKPK 351 LNKRVLPSKP KKSVKENLDE LSRRNIDVMH LQHILTNHLA FANVQQTPLF 401 QLQQVNSQIS ELSRDELRSI LSDAKCVGVI YRHGKDAAGK PLDEEYFYDL 451 ENDDDYERRN LVSSLKGGRT GLRSCRRTHK QYFWKKPAK*

Amino Acid Sequence (or in FASTA format)

       1  MSSQFPSSPY RTVDPYSPPN YKQQPNCPSS NYEKAGKTAS ESIGNFGKGD
      51  YPTPFPSSSI GRVSSPVRSN KVDAIPSSPA FPGQLAETSP KFSSKLSSPS
     101  RHTRVINAEL DPSKISTITV GRNSSQCDVA LCKNKFISRV HASITYLPQT
     151  NEVKIHCFSM NGLIVTYRKQ FDCYQLKDTM NNNNRAYRLV PRFSNEKCVK
     201  EIQDEGGFIN FTLEEGDTVY MTYYKGIMLD FRQVLLRISL KEKNSSSEPL
     251  RFEKKAEFES ESETKHMGSI RKHPLIFTDT SMDRPKKILK DSNKISIGSD
     301  SGVAERMLNH FLNSKSSPLS SVSSVDHEEQ TLRQDSLSSD KNPMTMKKPK
     351  LNKRVLPSKP KKSVKENLDE LSRRNIDVMH LQHILTNHLA FANVQQTPLF
     401  QLQQVNSQIS ELSRDELRSI LSDAKCVGVI YRHGKDAAGK PLDEEYFYDL
     451  ENDDDYERRN LVSSLKGGRT GLRSCRRTHK QYFWKKPAK*

external links for Tos4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tos4p

1)	Horak CE, et al. (2002) Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae. Genes Dev 16(23):3017-33
2)	de Lichtenberg U, et al. (2005) New weakly expressed cell cycle-regulated genes in yeast. Yeast 22(15):1191-201
3)	Tu BP, et al. (2005) Logic of the yeast metabolic cycle: temporal compartmentalization of cellular processes. Science 310(5751):1152-8
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Huang M and Elledge SJ (2000) The FHA domain, a phosphoamino acid binding domain involved in the DNA damage response pathway. Cold Spring Harb Symp Quant Biol 65():413-21
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41