SWI6/YLR182W Protein Information

Standard Name	Swi6p 1
Systematic Name	Ylr182wp
Alias	Psl8p , Sds11p 2
ORF Classification	Verified
Description	Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators (3, 4, 5, 6, 7, 8)
Name Description	SWItching deficient 1

Experimental Data
Molecules/cell	3340 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	803
Molecular Weight (Da)	90,559
Isoelectric Point (pI)	4.58

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Swi6p (InterPro)
Physical Interactions	There are 81 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001362A3 \| P09959 MIPS: YLR182W NCBI: 135078 \| 299688855 \| 299688856 \| 4598 \| 577200 \| 6137552 \| 6137553 \| 6323211 \| NP_013283.1 GenBank/EMBL/DDBJ: DAA09502.1 \| U17246 \| X06238

Amino Acid Sequence (or in FASTA format)
1 MALEEVVRYL GPHNEIPLTL TRDSETGHFL LKHFLPILQQ YHDTGNINET 51 NPDSFPTDEE RNKLLAHYGI AVNTDDRGEL WIELEKCLQL LNMLNLFGLF 101 QDAFEFEEPE TDQDEEDPSH SKLPENKTKS ENSKDNISSK RINNLQDMSL 151 DSDAHRELGS PLKKLKIDTS VIDAESDSTP NTARGKPNDD INKGPSGDNE 201 NNGTDDNDRT AGPIITFTHD LTSDFLSSPL KIMKALPSPV VNDNEQKMKL 251 EAFLQRLLFP EIQEMPTSLN NDSSNRNSEG GSSNQQQQHV SFDSLLQEVN 301 DAFPNTQLNL NIPVDEHGNT PLHWLTSIAN LELVKHLVKH GSNRLYGDNM 351 GESCLVKAVK SVNNYDSGTF EALLDYLYPC LILEDSMNRT ILHHIIITSG 401 MTGCSAAAKY YLDILMGWIV KKQNRPIQSG TNEKESKPND KNGERKDSIL 451 ENLDLKWIIA NMLNAQDSNG DTCLNIAARL GNISIVDALL DYGADPFIAN 501 KSGLRPVDFG AGTSKLQNTN GGDENSKMVS KGDYDGQKNG KAKKIRSQLL 551 KNPPETTSLI NDVQNLLNSI SKDYENETVQ YNEKLEKLHK ELNEQREELA 601 NSREQLANVK QLKDEYSLMQ EQLTNLKAGI EEEEESFREE SKKLGIIADE 651 SSGIDWDSSE YDADEPFKVE FLSDFLEDKL QKNYEGDISK LLEAESKEQI 701 MEQIRNQLPA EKIQSMLPPT VLLKARINAY KRNDKHLTNV LDTISTKQSE 751 LENKFRRVLS LCLKIDENKV DNMLDGLLQA ISSEDPQDID TDEMQDFLKK 801 HAS*

Amino Acid Sequence (or in FASTA format)

       1  MALEEVVRYL GPHNEIPLTL TRDSETGHFL LKHFLPILQQ YHDTGNINET
      51  NPDSFPTDEE RNKLLAHYGI AVNTDDRGEL WIELEKCLQL LNMLNLFGLF
     101  QDAFEFEEPE TDQDEEDPSH SKLPENKTKS ENSKDNISSK RINNLQDMSL
     151  DSDAHRELGS PLKKLKIDTS VIDAESDSTP NTARGKPNDD INKGPSGDNE
     201  NNGTDDNDRT AGPIITFTHD LTSDFLSSPL KIMKALPSPV VNDNEQKMKL
     251  EAFLQRLLFP EIQEMPTSLN NDSSNRNSEG GSSNQQQQHV SFDSLLQEVN
     301  DAFPNTQLNL NIPVDEHGNT PLHWLTSIAN LELVKHLVKH GSNRLYGDNM
     351  GESCLVKAVK SVNNYDSGTF EALLDYLYPC LILEDSMNRT ILHHIIITSG
     401  MTGCSAAAKY YLDILMGWIV KKQNRPIQSG TNEKESKPND KNGERKDSIL
     451  ENLDLKWIIA NMLNAQDSNG DTCLNIAARL GNISIVDALL DYGADPFIAN
     501  KSGLRPVDFG AGTSKLQNTN GGDENSKMVS KGDYDGQKNG KAKKIRSQLL
     551  KNPPETTSLI NDVQNLLNSI SKDYENETVQ YNEKLEKLHK ELNEQREELA
     601  NSREQLANVK QLKDEYSLMQ EQLTNLKAGI EEEEESFREE SKKLGIIADE
     651  SSGIDWDSSE YDADEPFKVE FLSDFLEDKL QKNYEGDISK LLEAESKEQI
     701  MEQIRNQLPA EKIQSMLPPT VLLKARINAY KRNDKHLTNV LDTISTKQSE
     751  LENKFRRVLS LCLKIDENKV DNMLDGLLQA ISSEDPQDID TDEMQDFLKK
     801  HAS*

external links for Swi6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Swi6p

1)	Breeden L and Nasmyth K (1987) Cell cycle control of the yeast HO gene: cis- and trans-acting regulators. Cell 48(3):389-97
2)	Sussel L, et al. (1995) Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure. Genetics 141(3):873-88
3)	Leem SH, et al. (1998) Meiotic role of SWI6 in Saccharomyces cerevisiae. Nucleic Acids Res 26(13):3154-8
4)	Ho Y, et al. (1999) Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein. Mol Cell Biol 19(8):5267-78
5)	Sidorova JM, et al. (1995) Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization. Mol Biol Cell 6(12):1641-58
6)	Horak CE, et al. (2002) Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae. Genes Dev 16(23):3017-33
7)	Scrimale T, et al. (2009) The Unfolded Protein Response Is Induced by the Cell Wall Integrity Mitogen-activated Protein Kinase Signaling Cascade and Is Required for Cell Wall Integrity in Saccharomyces cerevisiae. Mol Biol Cell 20(1):164-75
8)	Kim KY, et al. (2010) Yeast Mpk1 cell wall integrity mitogen-activated protein kinase regulates nucleocytoplasmic shuttling of the Swi6 transcriptional regulator. Mol Biol Cell 21(9):1609-19
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41