SAM1/YLR180W Protein Information

Standard Name	Sam1p 1
Systematic Name	Ylr180wp
Alias	Eth10p
ORF Classification	Verified
Description	S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	S-AdenosylMethionine requiring 6

Experimental Data
Molecules/cell	103000 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	382
Molecular Weight (Da)	41,818
Isoelectric Point (pI)	4.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sam1p (InterPro)
Physical Interactions	There are 114 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E07 \| P10659 MIPS: YLR180W NCBI: 1346525 \| 172534 \| 577201 \| 6323209 \| NP_013281.1 GenBank/EMBL/DDBJ: DAA09500.1 \| J03477 \| U17246
External Classifications	EC: 2.5.1.6 [Methionine adenosyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MAGTFLFTSE SVGEGHPDKI CDQVSDAILD ACLAEDPHSK VACETAAKTG 51 MIMVFGEITT KAQLDYQKIV RDTIKKIGYD DSAKGFDYKT CNVLVAIEQQ 101 SPDIAQGVHE EKDLEDIGAG DQGIMFGYAT DETPEGLPLT ILLAHKLNMA 151 MADARRDGSL AWLRPDTKTQ VTVEYKDDHG RWVPQRIDTV VVSAQHADEI 201 TTEDLRAQLK SEIIEKVIPR DMLDENTKYF IQPSGRFVIG GPQGDAGLTG 251 RKIIVDAYGG ASSVGGGAFS GKDYSKVDRS AAYAARWVAK SLVAAGLCKR 301 VQVQFSYAIG IAEPLSLHVD TYGTATKSDE EIIDIISKNF DLRPGVLVKE 351 LDLARPIYLP TASYGHFTNQ EYPWEKPKTL KF*

Amino Acid Sequence (or in FASTA format)

       1  MAGTFLFTSE SVGEGHPDKI CDQVSDAILD ACLAEDPHSK VACETAAKTG
      51  MIMVFGEITT KAQLDYQKIV RDTIKKIGYD DSAKGFDYKT CNVLVAIEQQ
     101  SPDIAQGVHE EKDLEDIGAG DQGIMFGYAT DETPEGLPLT ILLAHKLNMA
     151  MADARRDGSL AWLRPDTKTQ VTVEYKDDHG RWVPQRIDTV VVSAQHADEI
     201  TTEDLRAQLK SEIIEKVIPR DMLDENTKYF IQPSGRFVIG GPQGDAGLTG
     251  RKIIVDAYGG ASSVGGGAFS GKDYSKVDRS AAYAARWVAK SLVAAGLCKR
     301  VQVQFSYAIG IAEPLSLHVD TYGTATKSDE EIIDIISKNF DLRPGVLVKE
     351  LDLARPIYLP TASYGHFTNQ EYPWEKPKTL KF*

external links for Sam1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Sam1p

1)	Rosenberg, N. and Rothstein, R. (1992) Personal Communication, Mortimer Map Edition 11
2)	Thomas D and Surdin-Kerjan Y (1991) The synthesis of the two S-adenosyl-methionine synthetases is differently regulated in Saccharomyces cerevisiae. Mol Gen Genet 226(1-2):224-32
3)	Thomas D and Surdin-Kerjan Y (1987) SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. Sequence and expression. J Biol Chem 262(34):16704-9
4)	Kodaki T, et al. (2003) Differential transcriptional regulation of two distinct S-adenosylmethionine synthetase genes (SAM1 and SAM2) of Saccharomyces cerevisiae. Nucleic Acids Res Suppl(3):303-4
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Cherest H and Surdin-Kerjan Y (1978) S-adenosyl methionine requiring mutants in Saccharomyces cerevisiae: evidences for the existence of two methionine adenosyl transferases. Mol Gen Genet 163(2):153-67
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41