ASP3-3/YLR158C Protein Information

Standard Name	Asp3-3p
Systematic Name	Ylr158cp
Alias	Asp3p
ORF Classification	Verified
Description	Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-3 has a paralog, ASP3-1, that arose from a segmental duplication (1, 2, 3, 4, 5)
Name Description	ASParaginase

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	362
Molecular Weight (Da)	38,686
Isoelectric Point (pI)	4.53

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Asp3-3p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001260FD \| P0CX78 MIPS: YLR158C NCBI: 1234850 \| 338817580 \| 6323184 \| 6323186 \| 6323187 \| 6323189 \| NP_013256.1 \| NP_013258.1 \| NP_013259.1 \| NP_013261.1 GenBank/EMBL/DDBJ: DAA09478.1 \| U51921
External Classifications	EC: 3.5.1.1 [Asparaginase]

Amino Acid Sequence (or in FASTA format)
1 MRSLNTLLLS LFVAMSSGAP LLKIREEKNS SLPSIKIFGT GGTIASKGST 51 SATTAGYSVG LTVNDLIEAV PSLAEKANLD YLQVSNVGSN SLNYTHLIPL 101 YHGISEALAS DDYAGAVVTH GTDTMEETAF FLDLTINSEK PVCIAGAMRP 151 ATATSADGPM NLYQAVSIAA SEKSLGRGTM ITLNDRIASG FWTTKMNANS 201 LDTFRADEQG YLGYFSNDDV EFYYPPVKPN GWQFFDISNL TDPSEIPEVI 251 ILYSYQGLNP ELIVKAVKDL GAKGIVLAGS GAGSWTATGS IVNEQLYEEY 301 GIPIVHSRRT ADGTVPPDDA PEYAIGSGYL NPQKSRILLQ LCLYSGYGMD 351 QIRSVFSGVY GG*

Amino Acid Sequence (or in FASTA format)

       1  MRSLNTLLLS LFVAMSSGAP LLKIREEKNS SLPSIKIFGT GGTIASKGST
      51  SATTAGYSVG LTVNDLIEAV PSLAEKANLD YLQVSNVGSN SLNYTHLIPL
     101  YHGISEALAS DDYAGAVVTH GTDTMEETAF FLDLTINSEK PVCIAGAMRP
     151  ATATSADGPM NLYQAVSIAA SEKSLGRGTM ITLNDRIASG FWTTKMNANS
     201  LDTFRADEQG YLGYFSNDDV EFYYPPVKPN GWQFFDISNL TDPSEIPEVI
     251  ILYSYQGLNP ELIVKAVKDL GAKGIVLAGS GAGSWTATGS IVNEQLYEEY
     301  GIPIVHSRRT ADGTVPPDDA PEYAIGSGYL NPQKSRILLQ LCLYSGYGMD
     351  QIRSVFSGVY GG*

external links for Asp3-3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Asp3-3p

1)	Kim KW, et al. (1988) Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene. J Biol Chem 263(24):11948-53
2)	Oliveira EM, et al. (2003) The role of the GATA factors Gln3p, Nil1p, Dal80p and the Ure2p on ASP3 regulation in Saccharomyces cerevisiae. Yeast 20(1):31-7
3)	Katju V, et al. (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75
4)	Huang YC, et al. (2010) Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast. RNA 16(11):2085-93
5)	League GP, et al. (2012) The ASP3 locus in Saccharomyces cerevisiae originated by horizontal gene transfer from Wickerhamomyces. FEMS Yeast Res 12(7):859-63