SPE4/YLR146C Protein Information

Standard Name	Spe4p
Systematic Name	Ylr146cp
ORF Classification	Verified
Description	Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid (1, 2)
Name Description	SPErmidine auxotroph 3

Experimental Data
Molecules/cell	3870 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	300
Molecular Weight (Da)	34,090
Isoelectric Point (pI)	4.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Spe4p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E70 \| Q12455 MIPS: YLR146C NCBI: 1262306 \| 1360572 \| 3201943 \| 6094342 \| 62467581 \| 6323175 \| NP_013247.1 GenBank/EMBL/DDBJ: DAA09456.1 \| AF067970 \| AY899255 \| U53879 \| Z73318
External Classifications	EC: 2.5.1.22 [Spermine synthase]

Amino Acid Sequence (or in FASTA format)
1 MVNNSQHPYI KDGWFREIND KSFPGQAFTM TVDSILYEAR SEFQDILIFR 51 NKVYGTVLVL DGIVQCTEFD EFAYQEMITH IAMFAHSNPK RVLIIGGGDG 101 GVLREVAKHS CVEDITMVEI DSSVIELSRK FLPTLSNGAF DDERLDLKLC 151 DGFKFLQDIG ASDVHKKFDV IITDSSDPEG PAEAFFQERY FELLKDALNP 201 NGVVIMQSSE NFWLNLKYLH DLKNTAKKVF PNTEYCYTMV PTYTSGQLGL 251 IVCSNNANIP LNIPQRKISE QEQGKLKYYN PQIHSSAFVL PTWADKVINE 301 *

Amino Acid Sequence (or in FASTA format)

       1  MVNNSQHPYI KDGWFREIND KSFPGQAFTM TVDSILYEAR SEFQDILIFR
      51  NKVYGTVLVL DGIVQCTEFD EFAYQEMITH IAMFAHSNPK RVLIIGGGDG
     101  GVLREVAKHS CVEDITMVEI DSSVIELSRK FLPTLSNGAF DDERLDLKLC
     151  DGFKFLQDIG ASDVHKKFDV IITDSSDPEG PAEAFFQERY FELLKDALNP
     201  NGVVIMQSSE NFWLNLKYLH DLKNTAKKVF PNTEYCYTMV PTYTSGQLGL
     251  IVCSNNANIP LNIPQRKISE QEQGKLKYYN PQIHSSAFVL PTWADKVINE
     301  *

external links for Spe4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Spe4p

1)	Hamasaki-Katagiri N, et al. (1998) Spermine is not essential for growth of Saccharomyces cerevisiae: identification of the SPE4 gene (spermine synthase) and characterization of a spe4 deletion mutant. Gene 210(2):195-201
2)	White WH, et al. (2001) Saccharomyces cerevisiae is capable of de Novo pantothenic acid biosynthesis involving a novel pathway of beta-alanine production from spermine. J Biol Chem 276(14):10794-800
3)	Whitney PA and Morris DR (1978) Polyamine auxotrophs of Saccharomyces cerevisiae. J Bacteriol 134(1):214-20
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41