DPH6/YLR143W Protein Information Help

Standard Name Dph6p 1, 2
Systematic Name Ylr143wp
ORF Classification Verified
Description Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene (1, 2, 3, 4)
Name Description DiPHthamide biosynthesis 2
Experimental Data
Molecules/cell 2270 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 685
Molecular Weight (Da) 77,940
Isoelectric Point (pI) 5.75

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dph6p (InterPro)
Physical Interactions There are 4 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006AB7A | Q12429
MIPS: YLR143W
NCBI: 1256896 | 1360566 | 6323172 | 74676598 | 995714 | NP_013244.1
GenBank/EMBL/DDBJ: DAA09453.1 | U53881 | X91258 | Z73315
External Classifications EC: 6.3.1.14 [Diphthine--ammonia ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MKFIALISGG KDSFYNIFHC LKNNHELIAL GNIYPKESEE QELDSFMFQT
      51  VGHDLIDYYS KCIGVPLFRR SILRNTSNNV ELNYTATQDD EIEELFELLR
     101  TVKDKIPDLE AVSVGAILSS YQRTRVENVC SRLGLVVLSY LWQRDQAELM
     151  GEMCLMSKDV NNVENDTNSG NKFDARIIKV AAIGLNEKHL GMSLPMMQPV
     201  LQKLNQLYQV HICGEGGEFE TMVLDAPFFQ HGYLELIDIV KCSDGEVHNA
     251  RLKVKFQPRN LSKSFLLNQL DQLPVPSIFG NNWQDLTQNL PKQQAKTGEQ
     301  RFENHMSNAL PQTTINKTND KLYISNLQSR KSETVEKQSE DIFTELADIL
     351  HSNQIPRNHI LSASLLIRDM SNFGKINKIY NEFLDLSKYG PLPPSRACVG
     401  SKCLPEDCHV QLSVVVDVKN TGKEKINKNK GGLHVQGRSY WAPCNIGPYS
     451  QSTWLNDDAN QVSFISGQIG LVPQSMEILG TPLTDQIVLA LQHFDTLCET
     501  IGAQEKLLMT CYISDESVLD SVIKTWAFYC SNMNHRSDLW MDKSDDVEKC
     551  LVLVKISELP RGAVAEFGGV TCKRLIVDDN DSDKKEREEN DDVSTVFQKL
     601  NLNIEGFHNT TVSAFGYNRN FITGFVDSRE ELELILEKTP KSAQITLYYN
     651  PKEIITFHHH IGYYPVEKLF DYRGKEHRFG LHIRS*

external links for Dph6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Dph6p
1) Su X, et al.  (2012) Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc Natl Acad Sci U S A 109(49):19983-7
2) Uthman S, et al.  (2013) The Amidation Step of Diphthamide Biosynthesis in Yeast Requires DPH6, a Gene Identified through Mining the DPH1-DPH5 Interaction Network. PLoS Genet 9(2):e1003334
3) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41