SEN2/YLR105C Protein Information

Standard Name	Sen2p 1
Systematic Name	Ylr105cp
ORF Classification	Verified
Description	Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease (2)
Name Description	Splicing ENdonuclease 1
Gene Product	tRNA splicing endonuclease subunit 2

Experimental Data
Molecules/cell	319 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	377
Molecular Weight (Da)	44,109
Isoelectric Point (pI)	8.59

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sen2p (InterPro)
Physical Interactions	There are 15 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013584C \| P16658 MIPS: YLR105C NCBI: 1256853 \| 134427 \| 1360492 \| 172576 \| 6323134 \| NP_013206.1 GenBank/EMBL/DDBJ: DAA09421.1 \| M32336 \| U53876 \| Z73277
External Classifications	EC: 3.1.27.9 [tRNA-intron endonuclease]

Amino Acid Sequence (or in FASTA format)
1 MSKGRVNQKR YKYPLPIHPV DDLPELILHN PLSWLYWAYR YYKSTNALND 51 KVHVDFIGDT TLHITVQDDK QMLYLWNNGF FGTGQFSRSE PTWKARTEAR 101 LGLNDTPLHN RGGTKSNTET EMTLEKVTQQ RRLQRLEFKK ERAKLERELL 151 ELRKKGGHID EENILLEKQR ESLRKFKLKQ TEDVGIVAQQ QDISESNLRD 201 EDNNLLDENG DLLPLESLEL MPVEAMFLTF ALPVLDISPA CLAGKLFQFD 251 AKYKDIHSFV RSYVIYHHYR SHGWCVRSGI KFGCDYLLYK RGPPFQHAEF 301 CVMGLDHDVS KDYTWYSSIA RVVGGAKKTF VLCYVERLIS EQEAIALWKS 351 NNFTKLFNSF QVGEVLYKRW VPGRNRD*

Amino Acid Sequence (or in FASTA format)

       1  MSKGRVNQKR YKYPLPIHPV DDLPELILHN PLSWLYWAYR YYKSTNALND
      51  KVHVDFIGDT TLHITVQDDK QMLYLWNNGF FGTGQFSRSE PTWKARTEAR
     101  LGLNDTPLHN RGGTKSNTET EMTLEKVTQQ RRLQRLEFKK ERAKLERELL
     151  ELRKKGGHID EENILLEKQR ESLRKFKLKQ TEDVGIVAQQ QDISESNLRD
     201  EDNNLLDENG DLLPLESLEL MPVEAMFLTF ALPVLDISPA CLAGKLFQFD
     251  AKYKDIHSFV RSYVIYHHYR SHGWCVRSGI KFGCDYLLYK RGPPFQHAEF
     301  CVMGLDHDVS KDYTWYSSIA RVVGGAKKTF VLCYVERLIS EQEAIALWKS
     351  NNFTKLFNSF QVGEVLYKRW VPGRNRD*

external links for Sen2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sen2p

1)	Winey M and Culbertson MR (1988) Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae. Genetics 118(4):609-17
2)	Trotta CR, et al. (1997) The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases. Cell 89(6):849-58
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41