CDC45/YLR103C Protein Information

Standard Name	Cdc45p 1
Systematic Name	Ylr103cp
Alias	Sld4p 2
ORF Classification	Verified
Description	DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes (3, 4)
Name Description	Cell Division Cycle 1

Experimental Data
Molecules/cell	1730 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	650
Molecular Weight (Da)	74,248
Isoelectric Point (pI)	4.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cdc45p (InterPro)
Physical Interactions	There are 92 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531AC \| Q08032 MIPS: YLR103C NCBI: 1256852 \| 1360488 \| 1511658 \| 1572489 \| 2498209 \| 6323132 \| NP_013204.1 GenBank/EMBL/DDBJ: DAA09419.1 \| U53876 \| U56821 \| U65790 \| Z73275

Amino Acid Sequence (or in FASTA format)
1 MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK 51 KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGVI DLEAFLEIDP 101 QEYVIDTDEK SGEQSFRRDI YVLDAHRPWN LDNIFGSQII QCFDDGTVDD 151 TLGEQKEAYY KLLELDEESG DDELSGDEND NNGGDDEATD ADEVTDEDEE 201 DEDETISNKR GNSSIGPNDL SKRKQRKKQI HEYEGVLEEY YSQGTTVVNS 251 ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK 301 RLTPSSRNSV KTPDTLTLNI QPDYYLFLLR HSSLYDSFYY SNYVNAKLSL 351 WNENGKKRLH KMFARMGIPL STAQETWLYM DHSIKRELGI IFDKNLDRYG 401 LQDIIRDGFV RTLGYRGSIS ASEFVEALTA LLEVGNSTDK DSVKINNDNN 451 DDTDGEEEED NSAQKLTNLR KRWVSNFWLS WDALDDRKVE LLNRGIQLAQ 501 DLQRAIFNTG VAILEKKLIK HLRIYRLCVL QDGPDLDLYR NPLTLLRLGN 551 WLIECCAESE DKQLLPMVLA SIDENTDTYL VAGLTPRYPR GLDTIHTKKP 601 ILNNFSMAFQ QITAETDAKV RIDNFESSII EIRREDLSPF LEKLTLSGLL 651 *

Amino Acid Sequence (or in FASTA format)

       1  MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK
      51  KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGVI DLEAFLEIDP
     101  QEYVIDTDEK SGEQSFRRDI YVLDAHRPWN LDNIFGSQII QCFDDGTVDD
     151  TLGEQKEAYY KLLELDEESG DDELSGDEND NNGGDDEATD ADEVTDEDEE
     201  DEDETISNKR GNSSIGPNDL SKRKQRKKQI HEYEGVLEEY YSQGTTVVNS
     251  ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK
     301  RLTPSSRNSV KTPDTLTLNI QPDYYLFLLR HSSLYDSFYY SNYVNAKLSL
     351  WNENGKKRLH KMFARMGIPL STAQETWLYM DHSIKRELGI IFDKNLDRYG
     401  LQDIIRDGFV RTLGYRGSIS ASEFVEALTA LLEVGNSTDK DSVKINNDNN
     451  DDTDGEEEED NSAQKLTNLR KRWVSNFWLS WDALDDRKVE LLNRGIQLAQ
     501  DLQRAIFNTG VAILEKKLIK HLRIYRLCVL QDGPDLDLYR NPLTLLRLGN
     551  WLIECCAESE DKQLLPMVLA SIDENTDTYL VAGLTPRYPR GLDTIHTKKP
     601  ILNNFSMAFQ QITAETDAKV RIDNFESSII EIRREDLSPF LEKLTLSGLL
     651  *

external links for Cdc45p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Cdc45p

1)	Moir D, et al. (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
2)	Kamimura Y, et al. (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9
3)	Tye BK (1999) MCM proteins in DNA replication. Annu Rev Biochem 68:649-86
4)	McKie JM, et al. (1998) Direct selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene. Genome Res 8(8):834-41
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41