ERG27/YLR100W Protein Information

Standard Name	Erg27p
Systematic Name	Ylr100wp
ORF Classification	Verified
Description	3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs (1, 2, 3, 4)
Name Description	ERGosterol biosynthesis

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	347
Molecular Weight (Da)	39,724
Isoelectric Point (pI)	8.9

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Erg27p (InterPro)
Physical Interactions	There are 59 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006C374 \| Q12452 MIPS: YLR100W NCBI: 1256850 \| 1360483 \| 60392263 \| 6323129 \| NP_013201.1 GenBank/EMBL/DDBJ: DAA09417.1 \| U53876 \| Z73272
External Classifications	EC: 1.1.1.270 [3-beta-hydroxysteroid 3-dehydrogenase]

Amino Acid Sequence (or in FASTA format)
1 MNRKVAIVTG TNSNLGLNIV FRLIETEDTN VRLTIVVTSR TLPRVQEVIN 51 QIKDFYNKSG RVEDLEIDFD YLLVDFTNMV SVLNAYYDIN KKYRAINYLF 101 VNAAQGIFDG IDWIGAVKEV FTNPLEAVTN PTYKIQLVGV KSKDDMGLIF 151 QANVFGPYYF ISKILPQLTR GKAYIVWISS IMSDPKYLSL NDIELLKTNA 201 SYEGSKRLVD LLHLATYKDL KKLGINQYVV QPGIFTSHSF SEYLNFFTYF 251 GMLCLFYLAR LLGSPWHNID GYKAANAPVY VTRLANPNFE KQDVKYGSAT 301 SRDGMPYIKT QEIDPTGMSD VFAYIQKKKL EWDEKLKDQI VETRTPI*

Amino Acid Sequence (or in FASTA format)

       1  MNRKVAIVTG TNSNLGLNIV FRLIETEDTN VRLTIVVTSR TLPRVQEVIN
      51  QIKDFYNKSG RVEDLEIDFD YLLVDFTNMV SVLNAYYDIN KKYRAINYLF
     101  VNAAQGIFDG IDWIGAVKEV FTNPLEAVTN PTYKIQLVGV KSKDDMGLIF
     151  QANVFGPYYF ISKILPQLTR GKAYIVWISS IMSDPKYLSL NDIELLKTNA
     201  SYEGSKRLVD LLHLATYKDL KKLGINQYVV QPGIFTSHSF SEYLNFFTYF
     251  GMLCLFYLAR LLGSPWHNID GYKAANAPVY VTRLANPNFE KQDVKYGSAT
     301  SRDGMPYIKT QEIDPTGMSD VFAYIQKKKL EWDEKLKDQI VETRTPI*

external links for Erg27p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Erg27p

1)	Lees ND, et al. (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
2)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3)	Gachotte D, et al. (1999) Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation. Proc Natl Acad Sci U S A 96(22):12655-60
4)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press