CHA4/YLR098C Protein Information

Standard Name	Cha4p 1
Systematic Name	Ylr098cp
Alias	Sil2p 2 , Sil3p 2
ORF Classification	Verified
Description	DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain (1, 3)
Name Description	Catabolism of Hydroxy Amino acids 1, 4

Experimental Data
Molecules/cell	396 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	648
Molecular Weight (Da)	74,392
Isoelectric Point (pI)	6.95

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cha4p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000003EAAC \| P43634 MIPS: YLR098C NCBI: 1168927 \| 1256848 \| 1360479 \| 6323127 \| 886707 \| NP_013199.1 GenBank/EMBL/DDBJ: DAA09414.1 \| U53876 \| Z49975 \| Z73270

Amino Acid Sequence (or in FASTA format)
1 MMLEPSPPPL TTTVTPSLPS SLKKSVTDND QNNNNVPRKR KLACQNCRRR 51 RRKCNMEKPC SNCIKFRTEC VFTQQDLRNK RYSTTYVEAL QSQIRSLKEQ 101 LQILSSSSST IASNALSSLK NNSDHGDAPN EKILKYGETA QSALPSSESN 151 DENESDAFTK KMPSESPPPV GTNSIYPSNS LSIIKKKTDG STRYQQQQVS 201 LKNLSRSPLI LRSLSLFFKW LYPGHYLFIH RETFLSAFFG DTNTKSYYCS 251 EELVFAIAAL GSLISYKSET ELFQQSEVFY QRAKTIVLKK IFQLEDSSLA 301 ESSSSSKLAI IQTLLCLAFY DIGSGENPMA WYLSGLAFRI AHEIGLHLNP 351 EAWSNVYEDE LSIMDFEVRS RIYWGCYIAD HLIAILFGRS TSLRLSNSTV 401 PETDELPEIE TGIEEYIYDP KVILSTANPL KKLIVLSRIT EIFASKIFSP 451 NETLLQRSEY LAKFNLEVYN WRRDLPPELQ WTKRSLMEMT DFNPTIAYVW 501 FHYYIVLISY NKPFIYEIKQ SRELVEGYVD ELYYLLKVWK NKFKTFEKAT 551 IYMIYSAILA IQCMKSNLIK KDRKQDFLNF LSAPTLNYEL ARKFIENSED 601 ALHNSETMDL LGTLSHGNDF ALEYNFDFTL LNEIDMLIGG NTNDGLSK*

Amino Acid Sequence (or in FASTA format)

       1  MMLEPSPPPL TTTVTPSLPS SLKKSVTDND QNNNNVPRKR KLACQNCRRR
      51  RRKCNMEKPC SNCIKFRTEC VFTQQDLRNK RYSTTYVEAL QSQIRSLKEQ
     101  LQILSSSSST IASNALSSLK NNSDHGDAPN EKILKYGETA QSALPSSESN
     151  DENESDAFTK KMPSESPPPV GTNSIYPSNS LSIIKKKTDG STRYQQQQVS
     201  LKNLSRSPLI LRSLSLFFKW LYPGHYLFIH RETFLSAFFG DTNTKSYYCS
     251  EELVFAIAAL GSLISYKSET ELFQQSEVFY QRAKTIVLKK IFQLEDSSLA
     301  ESSSSSKLAI IQTLLCLAFY DIGSGENPMA WYLSGLAFRI AHEIGLHLNP
     351  EAWSNVYEDE LSIMDFEVRS RIYWGCYIAD HLIAILFGRS TSLRLSNSTV
     401  PETDELPEIE TGIEEYIYDP KVILSTANPL KKLIVLSRIT EIFASKIFSP
     451  NETLLQRSEY LAKFNLEVYN WRRDLPPELQ WTKRSLMEMT DFNPTIAYVW
     501  FHYYIVLISY NKPFIYEIKQ SRELVEGYVD ELYYLLKVWK NKFKTFEKAT
     551  IYMIYSAILA IQCMKSNLIK KDRKQDFLNF LSAPTLNYEL ARKFIENSED
     601  ALHNSETMDL LGTLSHGNDF ALEYNFDFTL LNEIDMLIGG NTNDGLSK*

external links for Cha4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Cha4p

1)	Holmberg S and Schjerling P (1996) Cha4p of Saccharomyces cerevisiae activates transcription via serine/threonine response elements. Genetics 144(2):467-78
2)	Pedersen JO, et al. (1997) Locus-specific suppression of ilv1 in Saccharomyces cerevisiae by deregulation of CHA1 transcription. Mol Gen Genet 255(6):561-9
3)	Schjerling P and Holmberg S (1996) Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators. Nucleic Acids Res 24(23):4599-607
4)	Petersen JG, et al. (1988) Molecular genetics of serine and threonine catabolism in Saccharomyces cerevisiae. Genetics 119(3):527-34
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41