SMC4/YLR086W Protein Information

Standard Name	Smc4p
Systematic Name	Ylr086wp
ORF Classification	Verified
Description	Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate (1, 2, 3)
Name Description	Structural Maintenance of Chromosomes

Experimental Data
Molecules/cell	573 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,418
Molecular Weight (Da)	162,188
Isoelectric Point (pI)	6.13

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Smc4p (InterPro)
Physical Interactions	There are 42 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005327B \| Q12267 MIPS: YLR086W NCBI: 1256888 \| 1360455 \| 2204269 \| 29427672 \| 6323115 \| NP_013187.1 \| NM_001181973.1 GenBank/EMBL/DDBJ: DAA09402.1 \| U53880 \| Z73258 \| Z73259

Amino Acid Sequence (or in FASTA format)
1 MSDSPLSKRQ KRKSAQEPEL SLDQGDAEED SQVENRVNLS ENTPEPDLPA 51 LEASYSKSYT PRKLVLSSGE NRYAFSQPTN STTTSLHVPN LQPPKTSSRG 101 RDHKSYSQSP PRSPGRSPTR RLELLQLSPV KNSRVELQKI YDRHQSSSKQ 151 QSRLFINELV LENFKSYAGK QVVGPFHTSF SAVVGPNGSG KSNVIDSMLF 201 VFGFRANKMR QDRLSDLIHK SEAFPSLQSC SVAVHFQYVI DESSGTSRID 251 EEKPGLIITR KAFKNNSSKY YINEKESSYT EVTKLLKNEG IDLDHKRFLI 301 LQGEVENIAQ MKPKAEKESD DGLLEYLEDI IGTANYKPLI EERMGQIENL 351 NEVCLEKENR FEIVDREKNS LESGKETALE FLEKEKQLTL LRSKLFQFKL 401 LQSNSKLAST LEKISSSNKD LEDEKMKFQE SLKKVDEIKA QRKEIKDRIS 451 SCSSKEKTLV LERRELEGTR VSLEERTKNL VSKMEKAEKT LKSTKHSISE 501 AENMLEELRG QQTEHETEIK DLTQLLEKER SILDDIKLSL KDKTKNISAE 551 IIRHEKELEP WDLQLQEKES QIQLAESELS LLEETQAKLK KNVETLEEKI 601 LAKKTHKQEL QDLILDLKKK LNSLKDERSQ GEKNFTSAHL KLKEMQKVLN 651 AHRQRAMEAR SSLSKAQNKS KVLTALSRLQ KSGRINGFHG RLGDLGVIDD 701 SFDVAISTAC PRLDDVVVDT VECAQHCIDY LRKNKLGYAR FILLDRLRQF 751 NLQPISTPEN VPRLFDLVKP KNPKFSNAFY SVLRDTLVAQ NLKQANNVAY 801 GKKRFRVVTV DGKLIDISGT MSGGGNHVAK GLMKLGTNQS DKVDDYTPEE 851 VDKIERELSE RENNFRVASD TVHEMEEELK KLRDHEPDLE SQISKAEMEA 901 DSLASELTLA EQQVKEAEMA YVKAVSDKAQ LNVVMKNLER LRGEYNDLQS 951 ETKTKKEKIK GLQDEIMKIG GIKLQMQNSK VESVCQKLDI LVAKLKKVKS 1001 ASKKSGGDVV KFQKLLQNSE RDVELSSDEL KVIEEQLKHT KLALAENDTN 1051 MNETLNLKVE LKEQSEQLKE QMEDMEESIN EFKSIEIEMK NKLEKLNSLL 1101 TYIKSEITQQ EKGLNELSIR DVTHTLGMLD DNKMDSVKED VKNNQELDQE 1151 YRSCETQDES EIKDAETSCD NYHPMNIDET SDEVSRGIPR LSEDELRELD 1201 VELIESKINE LSYYVEETNV DIGVLEEYAR RLAEFKRRKL DLNNAVQKRD 1251 EVKEQLGILK KKRFDEFMAG FNIISMTLKE MYQMITMGGN AELELVDSLD 1301 PFSEGVTFSV MPPKKSWRNI TNLSGGEKTL SSLALVFALH KYKPTPLYVM 1351 DEIDAALDFR NVSIVANYIK ERTKNAQFIV ISLRNNMFEL AQQLVGVYKR 1401 DNRTKSTTIK NIDILNRT*

Amino Acid Sequence (or in FASTA format)

       1  MSDSPLSKRQ KRKSAQEPEL SLDQGDAEED SQVENRVNLS ENTPEPDLPA
      51  LEASYSKSYT PRKLVLSSGE NRYAFSQPTN STTTSLHVPN LQPPKTSSRG
     101  RDHKSYSQSP PRSPGRSPTR RLELLQLSPV KNSRVELQKI YDRHQSSSKQ
     151  QSRLFINELV LENFKSYAGK QVVGPFHTSF SAVVGPNGSG KSNVIDSMLF
     201  VFGFRANKMR QDRLSDLIHK SEAFPSLQSC SVAVHFQYVI DESSGTSRID
     251  EEKPGLIITR KAFKNNSSKY YINEKESSYT EVTKLLKNEG IDLDHKRFLI
     301  LQGEVENIAQ MKPKAEKESD DGLLEYLEDI IGTANYKPLI EERMGQIENL
     351  NEVCLEKENR FEIVDREKNS LESGKETALE FLEKEKQLTL LRSKLFQFKL
     401  LQSNSKLAST LEKISSSNKD LEDEKMKFQE SLKKVDEIKA QRKEIKDRIS
     451  SCSSKEKTLV LERRELEGTR VSLEERTKNL VSKMEKAEKT LKSTKHSISE
     501  AENMLEELRG QQTEHETEIK DLTQLLEKER SILDDIKLSL KDKTKNISAE
     551  IIRHEKELEP WDLQLQEKES QIQLAESELS LLEETQAKLK KNVETLEEKI
     601  LAKKTHKQEL QDLILDLKKK LNSLKDERSQ GEKNFTSAHL KLKEMQKVLN
     651  AHRQRAMEAR SSLSKAQNKS KVLTALSRLQ KSGRINGFHG RLGDLGVIDD
     701  SFDVAISTAC PRLDDVVVDT VECAQHCIDY LRKNKLGYAR FILLDRLRQF
     751  NLQPISTPEN VPRLFDLVKP KNPKFSNAFY SVLRDTLVAQ NLKQANNVAY
     801  GKKRFRVVTV DGKLIDISGT MSGGGNHVAK GLMKLGTNQS DKVDDYTPEE
     851  VDKIERELSE RENNFRVASD TVHEMEEELK KLRDHEPDLE SQISKAEMEA
     901  DSLASELTLA EQQVKEAEMA YVKAVSDKAQ LNVVMKNLER LRGEYNDLQS
     951  ETKTKKEKIK GLQDEIMKIG GIKLQMQNSK VESVCQKLDI LVAKLKKVKS
    1001  ASKKSGGDVV KFQKLLQNSE RDVELSSDEL KVIEEQLKHT KLALAENDTN
    1051  MNETLNLKVE LKEQSEQLKE QMEDMEESIN EFKSIEIEMK NKLEKLNSLL
    1101  TYIKSEITQQ EKGLNELSIR DVTHTLGMLD DNKMDSVKED VKNNQELDQE
    1151  YRSCETQDES EIKDAETSCD NYHPMNIDET SDEVSRGIPR LSEDELRELD
    1201  VELIESKINE LSYYVEETNV DIGVLEEYAR RLAEFKRRKL DLNNAVQKRD
    1251  EVKEQLGILK KKRFDEFMAG FNIISMTLKE MYQMITMGGN AELELVDSLD
    1301  PFSEGVTFSV MPPKKSWRNI TNLSGGEKTL SSLALVFALH KYKPTPLYVM
    1351  DEIDAALDFR NVSIVANYIK ERTKNAQFIV ISLRNNMFEL AQQLVGVYKR
    1401  DNRTKSTTIK NIDILNRT*

external links for Smc4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Smc4p

1)	Stray JE and Lindsley JE (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28):26238-48
2)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
3)	Haeusler RA, et al. (2008) Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev 22(16):2204-14
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41