BUD20/YLR074C Protein Information Help

Standard Name Bud20p 1
Systematic Name Ylr074cp
ORF Classification Verified
Description C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593 (1, 2)
Name Description BUD site selection 1
Experimental Data
Molecules/cell 5630 3
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 166
Molecular Weight (Da) 18,517
Isoelectric Point (pI) 9.57

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Bud20p (InterPro)
Physical Interactions There are 135 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053221 | Q08004
MIPS: YLR074C
NCBI: 1360432 | 45270306 | 6323103 | 71152042 | NP_013175.1
GenBank/EMBL/DDBJ: DAA09391.1 | AY558208 | Z73246
Amino Acid Sequence (or in FASTA format)
 
       1  MGRYSVKRYK TKRRTRDLDL IYNDLSTKES VQKLLNQPLD ETKPGLGQHY
      51  CIHCAKYMET AIALKTHLKG KVHKRRVKEL RGVPYTQEVS DAAAGYNLNK
     101  FLNRVQEITQ SVGPEKESNE ALLKEHLDST LANVKTTEPT LPWAAADAEA
     151  NTAAVTEAES TASAST*

external links for Bud20p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome

YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Bud20p
1) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2) Bassler J, et al.  (2012) The conserved Bud20 zinc finger protein is a new component of the ribosomal 60S subunit export machinery. Mol Cell Biol 32(24):4898-912
3) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41