SHM2/YLR058C Protein Information Help

Standard Name Shm2p 1
Systematic Name Ylr058cp
Alias Shmt2p
ORF Classification Verified
Description Cytosolic serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis (1, 2)
Name Description Serine HydroxyMethyltransferase 1
Experimental Data
Molecules/cell 67600 3
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 469
Molecular Weight (Da) 52,218
Isoelectric Point (pI) 7.44

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Shm2p (InterPro)
Physical Interactions There are 37 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E5D | P37291
MIPS: YLR058C
NCBI: 1181279 | 1360402 | 1707995 | 439120 | 6323087 | NP_013159.1 | NM_001181945.1
GenBank/EMBL/DDBJ: AAA21023.1 | CAA64305.1 | CAA97588.1 | DAA09376.1 | L22529 | X94607 | Z73230
External Classifications EC: 2.1.2.1 [Glycine hydroxymethyltransferase]
Amino Acid Sequence (or in FASTA format)
       1  MPYTLSDAHH KLITSHLVDT DPEVDSIIKD EIERQKHSID LIASENFTST
      51  SVFDALGTPL SNKYSEGYPG ARYYGGNEHI DRMEILCQQR ALKAFHVTPD
     101  KWGVNVQTLS GSPANLQVYQ AIMKPHERLM GLYLPDGGHL SHGYATENRK
     151  ISAVSTYFES FPYRVNPETG IIDYDTLEKN AILYRPKVLV AGTSAYCRLI
     201  DYKRMREIAD KCGAYLMVDM AHISGLIAAG VIPSPFEYAD IVTTTTHKSL
     251  RGPRGAMIFF RRGVRSINPK TGKEVLYDLE NPINFSVFPG HQGGPHNHTI
     301  AALATALKQA ATPEFKEYQT QVLKNAKALE SEFKNLGYRL VSNGTDSHMV
     351  LVSLREKGVD GARVEYICEK INIALNKNSI PGDKSALVPG GVRIGAPAMT
     401  TRGMGEEDFH RIVQYINKAV EFAQQVQQSL PKDACRLKDF KAKVDEGSDV
     451  LNTWKKEIYD WAGEYPLAV*                                 

external links for Shm2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Aspergillus (AspGD) CYC2008 (complexes) MIPS YPL+
Candida (CGD) Complexome Pfam domains YeastGFP
YGOB DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Shm2p
1) McNeil JB, et al.  (1994) Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine. J Biol Chem 269(12):9155-65
2) Kastanos EK, et al.  (1997) Role of mitochondrial and cytoplasmic serine hydroxymethyltransferase isozymes in de novo purine synthesis in Saccharomyces cerevisiae. Biochemistry 36(48):14956-64
3) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41