RAD5/YLR032W Protein Information

Standard Name	Rad5p
Systematic Name	Ylr032wp
Alias	Rev2p 1 , Snm2p
ORF Classification	Verified
Description	DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7)
Name Description	RADiation sensitive

Experimental Data
Molecules/cell	1520 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,169
Molecular Weight (Da)	134,001
Isoelectric Point (pI)	6.29

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rad5p (InterPro)
Physical Interactions	There are 39 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013307E \| P32849 MIPS: YLR032W NCBI: 1360348 \| 172347 \| 257212 \| 417587 \| 6323060 \| NP_013132.1 \| NM_001181919.1 GenBank/EMBL/DDBJ: DAA09350.1 \| M96644 \| S46103 \| Z73204
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.-

Amino Acid Sequence (or in FASTA format)
1 MSHIEQEERK RFFNDDLDTS ETSLNFKSEN KESFLFANSH NDDDDDVVVS 51 VSDTTEGEGD RSIVPVRREI EEEGQNQFIT ELLRIIPEMP KDLVMELNEK 101 FGSQEEGLSL ALSHYFDHNS GTSISKIPSS PNQLNTLSDT SNSTLSPSSF 151 HPKRRRIYGF RNQTRLEDKV TWKRFIGALQ VTGMATRPTV RPLKYGSQMK 201 LKRSSEEISA TKVYDSRGRK KASMASLVRI FDIQYDREIG RVSEDIAQIL 251 YPLLSSHEIS FEVTLIFCDN KRLSIGDSFI LQLDCFLTSL IFEERNDGES 301 LMKRRRTEGG NKREKDNGNF GRTLTETDEE LESRSKRLAL LKLFDKLRLK 351 PILDEQKALE KHKIELNSDP EIIDLDNDEI CSNQVTEVHN NLRDTQHEEE 401 TMNLNQLKTF YKAAQSSESL KSLPETEPSR DVFKLELRNY QKQGLTWMLR 451 REQEFAKAAS DGEASETGAN MINPLWKQFK WPNDMSWAAQ NLQQDHVNVE 501 DGIFFYANLH SGEFSLAKPI LKTMIKGGIL SDEMGLGKTV AAYSLVLSCP 551 HDSDVVDKKL FDIENTAVSD NLPSTWQDNK KPYASKTTLI VVPMSLLTQW 601 SNEFTKANNS PDMYHEVYYG GNVSSLKTLL TKTKTPPTVV LTTYGIVQNE 651 WTKHSKGRMT DEDVNISSGL FSVNFYRIII DEGHNIRNRT TVTSKAVMAL 701 QGKCKWVLTG TPIINRLDDL YSLVKFLELD PWRQINYWKT FVSTPFESKN 751 YKQAFDVVNA ILEPVLLRRT KQMKDKDGKP LVELPPKEVV IKRLPFSKSQ 801 DLLYKFLLDK AEVSVKSGIA RGDLLKKYST ILVHILRLRQ VCCHPGLIGS 851 QDENDEDLSK NNKLVTEQTV ELDSLMRVVS ERFDNSFSKE ELDAMIQRLK 901 VKYPDNKSFQ SLECSICTTE PMDLDKALFT ECGHSFCEKC LFEYIEFQNS 951 KNLGLKCPNC RNQIDACRLL ALVQTNSNSK NLEFKPYSPA SKSSKITALL 1001 KELQLLQDSS AGEQVVIFSQ FSTYLDILEK ELTHTFSKDV AKIYKFDGRL 1051 SLKERTSVLA DFAVKDYSRQ KILLLSLKAG GVGLNLTCAS HAYMMDPWWS 1101 PSMEDQAIDR LHRIGQTNSV KVMRFIIQDS IEEKMLRIQE KKRTIGEAMD 1151 TDEDERRKRR IEEIQMLFE*

Amino Acid Sequence (or in FASTA format)

       1  MSHIEQEERK RFFNDDLDTS ETSLNFKSEN KESFLFANSH NDDDDDVVVS
      51  VSDTTEGEGD RSIVPVRREI EEEGQNQFIT ELLRIIPEMP KDLVMELNEK
     101  FGSQEEGLSL ALSHYFDHNS GTSISKIPSS PNQLNTLSDT SNSTLSPSSF
     151  HPKRRRIYGF RNQTRLEDKV TWKRFIGALQ VTGMATRPTV RPLKYGSQMK
     201  LKRSSEEISA TKVYDSRGRK KASMASLVRI FDIQYDREIG RVSEDIAQIL
     251  YPLLSSHEIS FEVTLIFCDN KRLSIGDSFI LQLDCFLTSL IFEERNDGES
     301  LMKRRRTEGG NKREKDNGNF GRTLTETDEE LESRSKRLAL LKLFDKLRLK
     351  PILDEQKALE KHKIELNSDP EIIDLDNDEI CSNQVTEVHN NLRDTQHEEE
     401  TMNLNQLKTF YKAAQSSESL KSLPETEPSR DVFKLELRNY QKQGLTWMLR
     451  REQEFAKAAS DGEASETGAN MINPLWKQFK WPNDMSWAAQ NLQQDHVNVE
     501  DGIFFYANLH SGEFSLAKPI LKTMIKGGIL SDEMGLGKTV AAYSLVLSCP
     551  HDSDVVDKKL FDIENTAVSD NLPSTWQDNK KPYASKTTLI VVPMSLLTQW
     601  SNEFTKANNS PDMYHEVYYG GNVSSLKTLL TKTKTPPTVV LTTYGIVQNE
     651  WTKHSKGRMT DEDVNISSGL FSVNFYRIII DEGHNIRNRT TVTSKAVMAL
     701  QGKCKWVLTG TPIINRLDDL YSLVKFLELD PWRQINYWKT FVSTPFESKN
     751  YKQAFDVVNA ILEPVLLRRT KQMKDKDGKP LVELPPKEVV IKRLPFSKSQ
     801  DLLYKFLLDK AEVSVKSGIA RGDLLKKYST ILVHILRLRQ VCCHPGLIGS
     851  QDENDEDLSK NNKLVTEQTV ELDSLMRVVS ERFDNSFSKE ELDAMIQRLK
     901  VKYPDNKSFQ SLECSICTTE PMDLDKALFT ECGHSFCEKC LFEYIEFQNS
     951  KNLGLKCPNC RNQIDACRLL ALVQTNSNSK NLEFKPYSPA SKSSKITALL
    1001  KELQLLQDSS AGEQVVIFSQ FSTYLDILEK ELTHTFSKDV AKIYKFDGRL
    1051  SLKERTSVLA DFAVKDYSRQ KILLLSLKAG GVGLNLTCAS HAYMMDPWWS
    1101  PSMEDQAIDR LHRIGQTNSV KVMRFIIQDS IEEKMLRIQE KKRTIGEAMD
    1151  TDEDERRKRR IEEIQMLFE*

external links for Rad5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rad5p

1)	Lemontt JF (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68(1):21-33
2)	Torres-Ramos CA, et al. (2002) Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol 22(7):2419-26
3)	Kiakos K, et al. (2002) Saccharomyces cerevisiae RAD5 influences the excision repair of DNA minor groove adducts. J Biol Chem 277(46):44576-81
4)	Blastyak A, et al. (2007) Yeast rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression. Mol Cell 28(1):167-75
5)	Carlile CM, et al. (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Kuang L, et al. (2013) A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5. DNA Repair (Amst) 12(1):27-37
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41