SNF7/YLR025W Protein Information Help

Standard Name Snf7p 1
Systematic Name Ylr025wp
Alias Did1p 2 , Vps32p 3, 4 , Rns4p 5 , Vpl5p 6
ORF Classification Verified
Description One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes (7, 8, 9)
Name Description Sucrose NonFermenting 10
Experimental Data
Molecules/cell 3270 11
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 240
Molecular Weight (Da) 26,987
Isoelectric Point (pI) 4.48

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Snf7p (InterPro)
Physical Interactions There are 119 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000135B2D | P39929
MIPS: YLR025W
NCBI: 1360334 | 45270294 | 6323053 | 730759 | NP_013125.1
GenBank/EMBL/DDBJ: DAA09343.1 | AY558202 | L09751 | Z73197
Amino Acid Sequence (or in FASTA format)
 
       1  MWSSLFGWTS SNAKNKESPT KAIVRLREHI NLLSKKQSHL RTQITNQENE
      51  ARIFLTKGNK VMAKNALKKK KTIEQLLSKV EGTMESMEQQ LFSIESANLN
     101  LETMRAMQEG AKAMKTIHSG LDIDKVDETM DEIREQVELG DEISDAISRP
     151  LITGANEVDE DELDEELDML AQENANQETS KIVNNNVNAA PISENKVSLP
     201  SVPSNKIKQS ENSVKDGEEE EDEEDEDEKA LRELQAEMGL *

external links for Snf7p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Snf7p
1) Vallier LG and Carlson M  (1991) New SNF genes, GAL11 and GRR1 affect SUC2 expression in Saccharomyces cerevisiae. Genetics 129(3):675-84
2) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
3) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
4) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
5) Unno K, et al.  (2005) Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae: Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles. FEMS Yeast Res 5(9):801-12
6) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
7) Babst M, et al.  (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17(11):2982-93
8) Babst M, et al.  (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82
9) Bowers K, et al.  (2004) Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic 5(3):194-210
10) Carlson M, et al.  (1981) Mutants of yeast defective in sucrose utilization. Genetics 98(1):25-40
11) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41