THI73/YLR004C Protein Information

Standard Name	Thi73p 1
Systematic Name	Ylr004cp
ORF Classification	Verified
Description	Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs (1, 2, 3, 4, 5)
Name Description	THI regulon 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	523
Molecular Weight (Da)	58,531
Isoelectric Point (pI)	8.26

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Thi73p (InterPro)
Transmembrane Domains	There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 11 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006A079 \| Q07904 MIPS: YLR004C NCBI: 1360292 \| 6323032 \| 74627236 \| NP_013104.1 GenBank/EMBL/DDBJ: DAA09322.1 \| Z73176
External Classifications	TC: 2.A.1.14 [The Anion: Cation Symporter (ACS) Family]

Amino Acid Sequence (or in FASTA format)
1 MKNMSQRSMD VEKKAANADS CSVSTSSINV DDADVALRFL KQNGLDESST 51 ANEDDVVAGE EANFYGSHEL SPKVLRKVDL FILPFLCCTY LLMFLDKALL 101 NYAASMGIKD HLKGNEFSNL GTIFSAAYIF MEPVVTYLIQ KFPISKILGT 151 FITVWGIVLA CHAACKTYAS LMVVRTLLGL FESSSAVGCI AISGMYYTKS 201 EQSARIGFWA TQAGTGYIVG GLISFGFLHY HGTAFTSWQI MFLVVGLVTV 251 AFGVLTFLYL PDNVTNAWFL NKEEKIQVVE HIRANQTGLE TKKFKKQQVK 301 ELFLHDKFTW PMLLLTACSQ ISTGAIGTFS VTITGTFGFD KYETALLQLP 351 IGAITAMIIL ITTQMLSRWG HITLITTSMY IPAIIGCIVL ISLPLSHKIG 401 NLFSLYLLYS GSCVITNIYI WNSCNTSGYT KRVFRNAITM IVYNVSCIIA 451 PQMFRAYSAP RYIPAKIALL VTQCVCVPLQ LYIGYICKKE NEKRDKEQEG 501 QERKKYQFLD LTDIENRNFR YIY*

Amino Acid Sequence (or in FASTA format)

       1  MKNMSQRSMD VEKKAANADS CSVSTSSINV DDADVALRFL KQNGLDESST
      51  ANEDDVVAGE EANFYGSHEL SPKVLRKVDL FILPFLCCTY LLMFLDKALL
     101  NYAASMGIKD HLKGNEFSNL GTIFSAAYIF MEPVVTYLIQ KFPISKILGT
     151  FITVWGIVLA CHAACKTYAS LMVVRTLLGL FESSSAVGCI AISGMYYTKS
     201  EQSARIGFWA TQAGTGYIVG GLISFGFLHY HGTAFTSWQI MFLVVGLVTV
     251  AFGVLTFLYL PDNVTNAWFL NKEEKIQVVE HIRANQTGLE TKKFKKQQVK
     301  ELFLHDKFTW PMLLLTACSQ ISTGAIGTFS VTITGTFGFD KYETALLQLP
     351  IGAITAMIIL ITTQMLSRWG HITLITTSMY IPAIIGCIVL ISLPLSHKIG
     401  NLFSLYLLYS GSCVITNIYI WNSCNTSGYT KRVFRNAITM IVYNVSCIIA
     451  PQMFRAYSAP RYIPAKIALL VTQCVCVPLQ LYIGYICKKE NEKRDKEQEG
     501  QERKKYQFLD LTDIENRNFR YIY*

external links for Thi73p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	TCDB
YGOB	DIP	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Thi73p

1)	Mojzita D and Hohmann S (2006) Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae. Mol Genet Genomics 276(2):147-61
2)	Llorente B and Dujon B (2000) Transcriptional regulation of the Saccharomyces cerevisiae DAL5 gene family and identification of the high affinity nicotinic acid permease TNA1 (YGR260w). FEBS Lett 475(3):237-41
3)	Paulsen IT, et al. (1998) Unified inventory of established and putative transporters encoded within the complete genome of Saccharomyces cerevisiae. FEBS Lett 430(1-2):116-25
4)	Epstein CB, et al. (2001) Genome-wide responses to mitochondrial dysfunction. Mol Biol Cell 12(2):297-308
5)	Palkova Z, et al. (2002) Ammonia pulses and metabolic oscillations guide yeast colony development. Mol Biol Cell 13(11):3901-14