COF1/YLL050C Protein Information Help

Standard Name Cof1p 1, 2
Systematic Name Yll050cp
ORF Classification Verified
Description Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes (1, 2, 3, 4, 5, 6, 7)
Name Description COFilin 1, 2
Gene Product cofilin 1, 2
Experimental Data
Molecules/cell 19600 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 143
Molecular Weight (Da) 15,901
Isoelectric Point (pI) 4.89

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cof1p (InterPro)
Physical Interactions There are 65 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000110C55 | Q03048
MIPS: YLL050C
NCBI: 1360251 | 157830663 | 2098523 | 2098524 | 263637 | 287600 | 3564 | 399275 | 5542421 | 6322978 | 642334 | NP_013050.1
GenBank/EMBL/DDBJ: DAA09274.1 | D13230 | S52662 | Z14971 | Z47973 | Z73155
Amino Acid Sequence (or in FASTA format)
 
       1  MSRSGVAVAD ESLTAFNDLK LGKKYKFILF GLNDAKTEIV VKETSTDPSY
      51  DAFLEKLPEN DCLYAIYDFE YEINGNEGKR SKIVFFTWSP DTAPVRSKMV
     101  YASSKDALRR ALNGVSTDVQ GTDFSEVSYD SVLERVSRGA GSH*

external links for Cof1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cof1p
1) Moon AL, et al.  (1993) Cofilin is an essential component of the yeast cortical cytoskeleton. J Cell Biol 120(2):421-35
2) Iida K, et al.  (1993) Isolation of a yeast essential gene, COF1, that encodes a homologue of mammalian cofilin, a low-M(r) actin-binding and depolymerizing protein. Gene 124(1):115-20
3) Ayscough KR, et al.  (1997) High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A. J Cell Biol 137(2):399-416
4) Moriyama K, et al.  (1996) Phosphorylation of Ser-3 of cofilin regulates its essential function on actin. Genes Cells 1(1):73-86
5) Theriot JA  (1997) Accelerating on a treadmill: ADF/cofilin promotes rapid actin filament turnover in the dynamic cytoskeleton. J Cell Biol 136(6):1165-8
6) Wriggers W, et al.  (1998) Cofilin and gelsolin segment-1: molecular dynamics simulation and biochemical analysis predict a similar actin binding mode. J Mol Biol 282(5):921-32
7) Curwin AJ, et al.  (2012) Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Mol Biol Cell 23(12):2327-38
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41