UBI4/YLL039C Protein Information

Standard Name	Ubi4p 1
Systematic Name	Yll039cp
Alias	Scd2p , Ub14p
ORF Classification	Verified
Description	Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description	Ubiquitin 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	381
Molecular Weight (Da)	42,826
Isoelectric Point (pI)	7.87

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ubi4p (InterPro)
Physical Interactions	There are 3398 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000007A9 \| P0CG63 MIPS: YLL039C NCBI: 109157081 \| 1326021 \| 1326022 \| 1360231 \| 159162804 \| 159162864 \| 159163450 \| 159164567 \| 159164727 \| 164519447 \| 195927471 \| 195927472 \| 288562761 \| 291463616 \| 291463622 \| 302595956 \| 326634394 \| 340780219 \| 422920073 \| 4734 \| 6322989 \| NP_013061.1 GenBank/EMBL/DDBJ: DAA09283.1 \| X01473 \| X01474 \| X05731 \| Z73144

Amino Acid Sequence (or in FASTA format)
1 MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL 51 EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVESSDTID 101 NVKSKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR 151 GGMQIFVKTL TGKTITLEVE SSDTIDNVKS KIQDKEGIPP DQQRLIFAGK 201 QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVESSDT 251 IDNVKSKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR 301 LRGGMQIFVK TLTGKTITLE VESSDTIDNV KSKIQDKEGI PPDQQRLIFA 351 GKQLEDGRTL SDYNIQKEST LHLVLRLRGG N*

Amino Acid Sequence (or in FASTA format)

       1  MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL
      51  EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVESSDTID
     101  NVKSKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR
     151  GGMQIFVKTL TGKTITLEVE SSDTIDNVKS KIQDKEGIPP DQQRLIFAGK
     201  QLEDGRTLSD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVESSDT
     251  IDNVKSKIQD KEGIPPDQQR LIFAGKQLED GRTLSDYNIQ KESTLHLVLR
     301  LRGGMQIFVK TLTGKTITLE VESSDTIDNV KSKIQDKEGI PPDQQRLIFA
     351  GKQLEDGRTL SDYNIQKEST LHLVLRLRGG N*

external links for Ubi4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ubi4p

1)	Ozkaynak E, et al. (1987) The yeast ubiquitin genes: a family of natural gene fusions. EMBO J 6(5):1429-39
2)	Shih SC, et al. (2000) Monoubiquitin carries a novel internalization signal that is appended to activated receptors. EMBO J 19(2):187-98
3)	Finley D, et al. (1994) Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol Cell Biol 14(8):5501-9
4)	Lucero P, et al. (2000) Monoubiquitination is sufficient to signal internalization of the maltose transporter in Saccharomyces cerevisiae. J Bacteriol 182(1):241-3
5)	Finley D, et al. (1987) The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses. Cell 48(6):1035-46
6)	Ozkaynak E, et al. (1984) The yeast ubiquitin gene: head-to-tail repeats encoding a polyubiquitin precursor protein. Nature 312(5995):663-6
7)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76