PRP19/YLL036C Protein Information

Standard Name	Prp19p 1, 2
Systematic Name	Yll036cp
Alias	Pso4p 3
ORF Classification	Verified
Description	Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain (4, 5)
Name Description	Pre-RNA Processing 1

Experimental Data
Molecules/cell	11700 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	503
Molecular Weight (Da)	56,569
Isoelectric Point (pI)	4.87

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prp19p (InterPro)
Physical Interactions	There are 339 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531EE \| P32523 MIPS: YLL036C NCBI: 1360227 \| 1524337 \| 1709753 \| 172272 \| 296863548 \| 39655045 \| 6322992 \| 88192421 \| 88192422 \| 88192423 \| 88192424 \| 88192425 \| 88192426 \| NP_013064.1 GenBank/EMBL/DDBJ: DAA09285.1 \| L09721 \| X99770 \| Z73142

Amino Acid Sequence (or in FASTA format)
1 MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI 51 VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWDA IMLENFKLRS 101 TLDSLTKKLS TVMYERDAAK LVAAQLLMEK NEDSKDLPKS SQQAVAITRE 151 EFLQGLLQSS RDFVARGKLK APKWPILKNL ELLQAQNYSR NIKTFPYKEL 201 NKSMYYDKWV CMCRCEDGAL HFTQLKDSKT ITTITTPNPR TGGEHPAIIS 251 RGPCNRLLLL YPGNQITILD SKTNKVLREI EVDSANEIIY MYGHNEVNTE 301 YFIWADNRGT IGFQSYEDDS QYIVHSAKSD VEYSSGVLHK DSLLLALYSP 351 DGILDVYNLS SPDQASSRFP VDEEAKIKEV KFADNGYWMV VECDQTVVCF 401 DLRKDVGTLA YPTYTIPEFK TGTVTYDIDD SGKNMIAYSN ESNSLTIYKF 451 DKKTKNWTKD EESALCLQSD TADFTDMDVV CGDGGIAAIL KTNDSFNIVA 501 LTP*

Amino Acid Sequence (or in FASTA format)

       1  MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI
      51  VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWDA IMLENFKLRS
     101  TLDSLTKKLS TVMYERDAAK LVAAQLLMEK NEDSKDLPKS SQQAVAITRE
     151  EFLQGLLQSS RDFVARGKLK APKWPILKNL ELLQAQNYSR NIKTFPYKEL
     201  NKSMYYDKWV CMCRCEDGAL HFTQLKDSKT ITTITTPNPR TGGEHPAIIS
     251  RGPCNRLLLL YPGNQITILD SKTNKVLREI EVDSANEIIY MYGHNEVNTE
     301  YFIWADNRGT IGFQSYEDDS QYIVHSAKSD VEYSSGVLHK DSLLLALYSP
     351  DGILDVYNLS SPDQASSRFP VDEEAKIKEV KFADNGYWMV VECDQTVVCF
     401  DLRKDVGTLA YPTYTIPEFK TGTVTYDIDD SGKNMIAYSN ESNSLTIYKF
     451  DKKTKNWTKD EESALCLQSD TADFTDMDVV CGDGGIAAIL KTNDSFNIVA
     501  LTP*

external links for Prp19p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Prp19p

1)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2)	Cheng SC, et al. (1993) PRP19: a novel spliceosomal component. Mol Cell Biol 13(3):1876-82
3)	Henriques JA, et al. (1989) PSO4: a novel gene involved in error-prone repair in Saccharomyces cerevisiae. Mutat Res 218(2):111-24
4)	Ohi MD, et al. (2003) Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat Struct Biol 10(4):250-5
5)	Ohi MD, et al. (2005) Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol 25(1):451-60
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41